中国临床药理学杂志2024，Vol.40Issue(10)：1429-1433,5.DOI:10.13699/j.cnki.1001-6821.2024.10.008
槲皮素通过调控GAS5/miR-18b-5p轴抑制神经内分泌肿瘤细胞活性的研究
Quercetin inhibits the activity of neuroendocrine tumor cells by regulating the GAS5/miR-18b-5p axis
摘要
Abstract
Objective To investigate the inhibitory effect of quercetin on Gastro entero pancreatic NEN(GEP-NEN).Methods Human pancreatic neuroendocrine tumor BON-1 cells were randomly divided into control group,quercetin group(80 μmol·L-1 quercetin),quercetin+si-NC group(transfected with si-NC+80 μmol·L-1 quercetin),quercetin+si-growth arrest-specific+ranscript 5(GAS5)group(transfected with si-GAS5+80 μmol·L-1 quercetin).Dual luciferase reporter gene assay was used to verify the targeted binding of GASS5 to miR-18b-5p;real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the mRNA expression levels of B-cell lymphoma-2(Bcl-2)and Bel-2 associated X protein(Bax);positive expression of GAS5 and miR-18b-5p in cells was detected by fluorescence in situ hybridization(FISH)assay.Results Dual luciferase reporter gene results showed that GAS5 was targeted to miR-18b-5p.The GAS5 expression levels of control group,quercetin group,quercetin+si-NC group and quercetin+si-GAS5 group were 1.00±0.13,1.72±0.19,1.78±0.14 and 1.16±0.11,respectively;the expression levels of miR-18b-5p were 1.00±0.15,0.67±0.08,0.72±0.06 and 0.95±0.11 respectively;Bax mRNA expression levels were 1.00±0.12,2.17±0.25,2.32±0.28 and 1.37±0.15,respectively;Bcl-2 mRNA expression levels were 1.00±0.15,0.41±0.05,0.37±0.06 and 1.21±0.13,respectively.The above indexes were significantly different between quercetin group and control group(all P＜0.05);the above indexes were significantly different between quercetin+si-NC group and quercetin+si-GAS5 group(all P＜0.05).Conclusion Quercetin may slow down the development of GEP-NEN by targeting GAS5/miR-18b-5p molecular axis to inhibit cell growth.关键词
槲皮素/胃肠胰神经内分泌肿瘤/胰腺神经内分泌瘤细胞/生长抑制特异性基因5/细胞活性Key words
quercetin/gastro entero pancreatic neuroendocrine neoplasm/pancreatic neuroendocrine tumor cells/growth inhibition specific gene 5/cell activity分类
医药卫生引用本文复制引用
武文娟,李波,吕海宏,陈军,寇温..槲皮素通过调控GAS5/miR-18b-5p轴抑制神经内分泌肿瘤细胞活性的研究[J].中国临床药理学杂志,2024,40(10):1429-1433,5.基金项目
甘肃省科研计划基金资助项目(23JRRA0946) （23JRRA0946）
甘肃省科研计划基金资助项目(18JR3RA341) （18JR3RA341）
