中国临床药理学杂志2024，Vol.40Issue(10)：1434-1437,4.DOI:10.13699/j.cnki.1001-6821.2024.10.009
丹酚酸B调节脐带间充质干细胞抗氧化应激能力的作用研究
Effects of salvianolic acid B on anti-oxidative stress of umbilical cord mesenchymal stem cells
摘要
Abstract
Objective To investigate the effect of salvianolic acid B on the antioxidant stress capacity of human umbilical cord mesenchymal stem cells(HUCMSCs)and to improve the clinical application efficiency of mesenchymal stem cells.Methods The 5th generation HUMSCs were used for the experiment and divided into control group,model group,and experimental-L,-M,-H groups.The control group was cultured normally;the model group was treated with 800 μmol·L-1 hydrogen peroxide(H2 O2)for 2 h;and the experimental-L,-M,-H groups were pretreated with 2,10,20 μg·mL-1salvianolic acid B for 24 h before adding 800 μmol·L-1 H2 O2 for 2 h.The levels of glutathione(GSH),superoxide dismutase(SOD),and malondialdehyde(MDA)in the cell supernatant of each group were detected using test kits;real-time fluorescence quantitative reverse transcription polymerase chain reaction was used to detect the mRNA expression of apoptosis-related genes Caspase 1,and B-cell lymphoma-2(Bcl-2).Results After treatment with 800 μmol·L-1H2 O2 for 2 h,the levels of MDA in the cell supernatants of the control group,model group,and experimental-H group were(3.27±0.41),(6.50±0.21)and(4.79±0.40)nmol·mL-1,respectively;the GSH levels were(35.43±0.72),(20.13±0.58)and(32.30±3.87)μmoL·L-1;the SOD levels were(5.34±0.18),(3.34±0.20)and(5.09±0.15)U·mL-1;the expression of Caspase 1 mRNA were 1.02±0.21,2.78±0.26 and 2.37±0.32;the expression of Bcl-2 mRNA were 1.01±0.12,0.43±0.03 and 0.60±0.17.Compared with the control group,the above indexes in the model group were statistically significant(P＜0.05,P＜0.01).Compared with the model group,the above indexes in the experimental-H group were statistically significant(P＜0.05,P＜0.01).Conclusion Salvianolic acid B can reduce apoptosis caused by oxidative stress and enhance the antioxidant stress capacity of HUCMSCs.关键词
丹酚酸B/脐带间充质干细胞/氧化应激/凋亡/心血管疾病Key words
salvianolic acid B/umbilical cord mesenchymal stem cells/oxidative stress/apoptosis/cardiovascular disease分类
医药卫生引用本文复制引用
栾玉玲,张凌霄,丁新月,刘宗军..丹酚酸B调节脐带间充质干细胞抗氧化应激能力的作用研究[J].中国临床药理学杂志,2024,40(10):1434-1437,4.基金项目
上海市"科技创新行动计划"医学创新研究专项基金资助项目(21Y11909600) （21Y11909600）
