中国临床药理学杂志2024，Vol.40Issue(10)：1453-1457,5.DOI:10.13699/j.cnki.1001-6821.2024.10.013
当归黄芪胶囊对心力衰竭大鼠心肌自噬的影响
Effects of Angelica Sinensis and Astragalus capsules on myocardial autophagy in rats with heart failure
摘要
Abstract
Objective To investigate whether Angelica Sinensis and Astragalus capsules(AAC)regulates myocardial autophagy in heart failure rats via the phosphatidylinositol 3 kinase(PI3K)/protein kinase(Akt)/mammalian target of sirolimus(mTOR)signaling pathway.Methods A rat model of heart failure was constructed by intraperitoneal 2.5 mg·kg-1 doxorubicin,and another 8 rats served as the control group.The modeling rats were randomly divided into model group,control group and experimental-L,-M,-H groups.Control group was given 30 mg·kg-1 3-methyladenine by intraperitoneal injection;experimental-L,-M,-H groups were given 150,300 and 450 mg·kg-1 AAC by gavage,respectively;blank and model groups were given the same quantity of sterile distilled water.Six groups were administered once daily for 6 weeks.The cardiac function was measured by ultrasound,and the expression levels of PI3K,Akt,mTOR,sequestosome 1(P62)and microtubule-associated light chain protein 3-Ⅱ/Ⅰ(LC3 Ⅱ/Ⅰ)in myocardial tissue were measured by Western blot.Results In the blank,model,control and experimental-H groups,the left ventricular ejection fraction values were(85.00±3.63)％,(56.75±4.83)％,(75.63±3.70)％and(72.75±4.23)％;the relative expression levels of PI3K were 1.00±0,0.28±0.05,0.64±0.08 and 0.74±0.16;phosphorylated Akt/Akt were 1.00±0,0.49±0.06,0.90±0.16 and 0.95±0.10;phosphorylated mTOR/mTOR values were 1.00±0,0.42±0.09,0.73±0.13 and 0.83±0.08;the relative expression levels of P62 proteins were 1.00±0,0.24±0.12,0.57±0.09 and 0.96±0.10;the relative expression levels of LC3 Ⅱ/Ⅰ proteins were 1.00±0,4.31±0.75,2.20±0.76 and 1.59±0.24,respectively.Compared to the model group,statistical significant were identified in the experimental-H and control groups(all P＜0.05).Conclusion AAC can regulate PI3K/Akt/mTOR pathway,inhibit myocardial autophagy and improve cardiac function in rats with heart failure.关键词
当归黄芪胶囊/心力衰竭/自噬/磷脂酰肌醇3-激酶/蛋白激酶B/西罗莫司靶蛋白/微管相关轻链蛋白3 Ⅱ/ⅠKey words
Angelica sinensis and Astragalus capsule/heart failure/autophagy/phosphatidylinositol 3 kinase/protein kinase B/mammalian target of sirolimus/microtubule associated light chain protein 3-Ⅱ/Ⅰ分类
中医学引用本文复制引用
吴雪,吕欣芳,支晓东,赵信科,李应东..当归黄芪胶囊对心力衰竭大鼠心肌自噬的影响[J].中国临床药理学杂志,2024,40(10):1453-1457,5.基金项目
中医药防治重大疾病科研课题基金资助项目(ZGKZD-2018-2) （ZGKZD-2018-2）
甘肃省科技重大专项计划基金资助项目(20ZD7FA002) （20ZD7FA002）
甘肃省科技计划基金资助项目(21JR7RA399) （21JR7RA399）
甘肃省教育厅揭榜挂帅基金资助项目(2021jyjbgs-03) （2021jyjbgs-03）
兰州市科技局基金资助项目(2023-QN-191,2018-ZD-1) （2023-QN-191,2018-ZD-1）
兰州大学第二医院萃英科技创新基金资助项目(CY2021-BJ-A17) （CY2021-BJ-A17）
