中国临床药理学杂志2024，Vol.40Issue(10)：1458-1462,5.DOI:10.13699/j.cnki.1001-6821.2024.10.014
髓样分化蛋白-2调控AMPK介导肥胖所致心肌损伤的研究
Medullary differentiation-2 mediates obesity-induced myocardial injury by regulating adenosine AMPK
摘要
Abstract
Objective To investigate the effects of medullary differentiation-2(MD-2)in obesity-induced myocardial injury by regulating adenosine 5'-monophosphate-activated protein kinase(AMPK)activity.Methods The obese mice model was established by feeding high-fat feed.The wild-type and MD-2 gene knockout mice were randomly divided into wild-type(WT)-control group(fed normally),WT-model group(fed with high-fat diet),knockout(KO)-control group(fed normally)and KO-model group(fed with high-fat diet)with 8 mice in each group.At week 16,creatine kinase-MB(CK-MB)and lactate dehydrogenase(LDH)were measured by reagent kits;the left ventricular ejection fraction(LVEF)and left ventricular fraction shortening(LVFS)were measured with ultrasonic apparatus;the pathological changes in myocardial tissue were observed by hematoxylin and eosin(HE)staining and Masson staining;the protein expression of phosphorylation AMPK(p-AMPK)was measured by Western blot.Results HE staining and Masson staining results revealed significant necrosis and fibrosis of the myocardial tissue in WT-model group,while the degrees of necrosis and fibrosis in KO-model group were significantly reduced.The LVEF values of the WT-control group,WT-model group,KO-control group and KO-model group were(83.98±7.58)％,(52.03±4.91)％,(76.42±3.89)％and(73.71±4.22)％,respectively;the LVFS values of each group were(53.61±8.51)％,(24.56±3.76)％,(48.14±5.00)％and(44.07±3.74)％,respectively;the CK-MB values of each group were(49.83±15.49),(83.75±11.90),(54.03±18.66)and(66.85±12.98)U·L-1,respectively;the LDH values of each group were(12.24±3.20),(27.90±6.10),(13.55±3.55)and(15.53±2.58)U·L-1,respectively;the relative expression levels of p-AMPK in each group were 221.31±88.76,46.29±10.07,148.66±32.02 and 161.49±78.11.Compared with WT-control group,the above indicators in WT-model group,compared with WT-model group,the above indicators in KO-model group,the differences were statistically significant(all P＜0.05).Conclusion MD-2 mediates high-fat feed induced myocardial injury in obese mice by inhibiting AMPK activity.关键词
髓样分化蛋白-2/AMP依赖的蛋白激酶/肥胖/心肌损伤Key words
medullary differentiation-2/adenosine 5'-monophosphate-activated protein kinase/obesity/myocardial injury分类
医药卫生引用本文复制引用
黄成坷,周伶俐,孙未,林晓东,陈瑞杰..髓样分化蛋白-2调控AMPK介导肥胖所致心肌损伤的研究[J].中国临床药理学杂志,2024,40(10):1458-1462,5.基金项目
温州市科技计划基金资助项目(Y2020969) （Y2020969）
