斜纹夜蛾高致病力生防真菌的筛选与鉴定OA北大核心CSTPCD
Screening and Identification of Biocontrol Fungus with High Pathogenicity against Spodoptera litura
本研究从斜纹夜蛾幼虫僵虫中分离并纯化虫生真菌,得到 6株昆虫病原真菌,利用形态学和分子生物学方法对上述真菌进行鉴定,采用浸渍法测定 6 株真菌对斜纹夜蛾 2 龄幼虫的毒力.结果表明,6 株虫生真菌分别为2株球孢白僵菌Beauveria bassiana,以及莱氏绿僵菌Metarhizium rileyi,木贼镰刀菌Fusarium equiseti,曲霉菌Aspergillus nomiae和糙孢蓝状菌Talaromyces trachyspermus各 1株.在 1×108个孢子/mL浓度下,LT50分别为 4.737 d,4.543 d,1.343 d,2.086 d,2.212 d和 2.351 d,6株虫生真菌对斜纹夜蛾 2龄幼虫均表现出较强致病力,其中莱氏绿僵菌表现最好.本研究首次报道了镰刀菌和曲霉菌能够感染斜纹夜蛾,并首次发现糙孢蓝状菌能够感染昆虫.
Six strains of entomogenous fungi were isolated and purified from muscardine cadavers of Spodoptera litura larvae,and were identified by morphological and molecular biological methods.The pathogenicity of these strains against S.litura larvae was determined by the dipping method.The six fungal strains were identified as Beauveria bassiana(two strains),Metarhizium rileyi,Fusarium equiseti,Aspergillus nomiae,and Talaromyces trachyspermus,whose LT50 against the pest larvae were 4.737,4.543,1.343,2.086,2.212,and 2.351 days,respectively.All the six strains of entomogenous fungi showed high pathogenicity against the pests at the concentration of 108 conidia/mL,in which M.rileyi established the highest pathogenicity.The present study reports for the first time that S.litura can be infected by F.equiseti and A.nomiae and that T.trachyspermus is able to infect insects.
田艺帆;张正坤;隋丽;赵宇;路杨;孟钊;石旺鹏;李启云
吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室,长春 130033||中国农业大学植物保护学院,北京 100083吉林省农业科学院植物保护研究所/农业农村部东北作物有害生物综合治理重点实验室/吉林省农业微生物重点实验室,长春 130033公主岭市气象局,公主岭 136100中国农业大学植物保护学院,北京 100083
植物保护学
斜纹夜蛾虫生真菌毒力
Spodoptera lituraentomogenous fungipathogenicity
《中国生物防治学报》 2024 (002)
282-290 / 9
吉林省科技厅中青年科技创新创业卓越人才(团队)项目(创新类)(20230508011RC)
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