东北大黑鳃金龟幼虫高毒力球孢白僵菌菌株的筛选及应用OA北大核心CSTPCD

The aim of this study was to screen for highly toxicity Beauveria bassianan strains against the larvae of Holotrichia diomphalia Bates.The pathogenicity of a B.bassiana strain BbQCGZL-1 against the white grubs was determined by laboratory bioassay and its control efficiency against the grubs was compared to other agents.Further,the control efficiency of B.bassiana BbQCGZL-1 against the white grubs on different crops was tested by using outdoor potted plants.The results showed that the corrected mortality rate of the grubs was more than 75%at 14 d post treatment with the B.bassiana BbQCGZL-1,higher than those obtained with other stains tested;The LC50 was 1.21×107 cfu/mL at 7 d post treatment,the LT50 was 8.82 d with a concentration of spore suspension of 1.0×108 cfu/mL.The results of the laboratory bioassay and the outdoor potted plant experiment showed no significant difference in the control efficiency between B.bassiana BbQCGZL-1 and commercial biological agents against the white grubs in Northeast of China.Overall,the B.bassiana BbQCGZL-1 can be used as a microbial agent to control H.diomphalia larvae in the field.