中国生物防治学报2024，Vol.40Issue(2)：310-318,9.DOI:10.16409/j.cnki.2095-039x.2024.01.002
亚洲玉米螟越冬后幼虫致病细菌——黏质沙雷氏菌的分离鉴定及杀虫活性评价
Isolation,Identification and Evaluation of Insecticidal Activity of an Entomopathogenic Bacteria Strain Infecting Post-overwintering Ostrinia furnacalis Larvae
摘要
Abstract
A bacterial strain ACB3014 was isolated from the infected overwintering larvae of Ostrinia furnacalis in spring sown corn fields in Xinyuan County,Yili Valley,Xinjiang and identified by morphological observation,physiological and biochemical characteristics and 16S rDNA sequence analysis.The pathogenicity of this strain against the 3rd instar larvae of O.furnacalis was determined by feeding method.The strain ACB3014 was Serratia marcescens.The cumulative corrected mortality of ACB3014 on the 3rd instar larvae increased with the increase of bacterial suspension concentration.On the 7th day after feeding ACB3014 bacterial suspension,the cumulative adjusted mortality of the 3rd instar larvae ranged between 27.78%—77.78%at the concentration of 2×104-2×108 cfu/mL.On the 13th day of inoculation,when the concentration reached 2×108 cfu/mL,LC50 and LT50 against the 3rd instar larvae of O.furnacalis were 3.79×103 cfu/mL and 3.93 days,respectively.In addition,the infected O.furnacalis larvae are dark red and black and are pyoid and festering,giving off a strong odor.In conclusion,ACB3014 strain has good insecticidal activity against O.furnacalis larvae and has a high potential as a green biological control resource for O.furnacalis.关键词
亚洲玉米螟/黏质沙雷氏菌/毒力测定/致病力/杀虫活性Key words
Ostrinia furnacalis/Serratia marcescens/determination of virulence/pathogenic/insecticidal activity分类
农业科技引用本文复制引用
袁梓涵,王小武,丁新华,付开赟,蒋旭东,巩雪花,贾尊尊,吐尔逊·阿合买提,郭文超..亚洲玉米螟越冬后幼虫致病细菌——黏质沙雷氏菌的分离鉴定及杀虫活性评价[J].中国生物防治学报,2024,40(2):310-318,9.基金项目
新疆维吾尔自治区重大科技专项 （）
新疆农业科学院稳定支持专项(xjnkywdzc-2022004) （xjnkywdzc-2022004）
新疆玉米产业技术体系(XJARS-02) （XJARS-02）
