中国药房2024，Vol.35Issue(10)：1186-1192,7.DOI:10.6039/j.issn.1001-0408.2024.10.06
益气活血方对大鼠腰椎间盘突出的影响及机制
Effects and mechanism of Yiqi huoxue decoction on lumbar disc herniation in rats
摘要
Abstract
OBJECTIVE To investigate the effects and mechanism of Yiqi huoxue decoction(YQHX)on lumbar disc herniation in rats.METHODS Rats were randomly divided into sham operation group,model group,NF-κB inhibitor group(QNZ group,1 mg/kg),YQHX group(9.1 g/kg)and combination group(YQHX+QNZ group,the same dose as each single drug group),with 10 rats in each group.Except for sham operation group,lumbar disc herniation model of rats was induced in other groups;administration groups were given QNZ intraperitoneally or/and YQHX intragastrically,once a day,for 8 consecutive weeks.The severity of intervertebral disc herniation was evaluated in each group;the pathological changes of intervertebral discs and the changes of autophagy of nucleus pulposus cells were all observed;the level of tumor necrosis factor-α(TNF-α)in serum,and the ratios of Bcl-2/adenovirus E1B interacting protein 3(BNIP3)and Beclin-1 positive cells in intervertebral disc tissues were detected;the phosphorylation of nuclear factor-κB(NF-κB)p65,the expressions of tumor necrosis factor receptor-associated factor-2(TRAF-2),TRAF-3,BNIP3 and LC3B protein,and mRNA expressions of NF-κB p65,LC3B,p62,BNIP3 and Beclin-1 were determined.RESULTS Compared with model group,Pfirrmann grading score decreased significantly,the pathological injury of intervertebral disc tissue was relieved in YQHX group;the number of autophagosomes in nucleus pulposus cells increased;serum level of TNF-α and mRNA expression of p62 in intervertebral disc tissue decreased significantly;the ratios of BNIP3 and Beclin-1 positive cells,the phosphorylation of NF-κB p65,the expressions of TRAF-2,TRAF-3,BNIP3 and LC3B protein as well as the mRNA expressions of NF-κB p65,LC3B,BNIP3 and Beclin-1 decreased significantly in intervertebral disc tissues(P＜0.05).The changes of above indexes in YQHX group were reversed partly in YQHX+QNZ group.CONCLUSIONS YQHX promotes the elevation of autophagy level of intervertebral discs,slows down the inflammatory response and the progression of lumbar disc herniation by activating the NF-κB signaling pathway.关键词
腰椎间盘突出/炎症反应/NF-κB信号通路/自噬Key words
lumbar disc herniation/inflammatory response/NF-κB signaling pathway/autophagy分类
医药卫生引用本文复制引用
白雪,刘锦涛,孙孝先,郭杨,马勇,刘孟敏,姜宏,林顺,曹瑞,袁永丰..益气活血方对大鼠腰椎间盘突出的影响及机制[J].中国药房,2024,35(10):1186-1192,7.基金项目
国家自然科学基金面上项目(No.82074467,No.82374220) （No.82074467,No.82374220）
国家中医药管理局高水平中医药重点学科建设项目(No.国中医药人教函[2023]85号) （No.国中医药人教函[2023]85号）
江苏省中医药领军人才培养项目(No.苏中医科教[2023]17号) （No.苏中医科教[2023]17号）
江苏省研究生科研与实践创新计划项目(No.KYCX23_2202) （No.KYCX23_2202）
