姜黄素纳米胶束的制备、表征及抗酒精性肝病体外活性评价OA北大核心CSTPCD

OBJECTIVE To prepare and characterize curcumin nanomicelles(hereinafter referred to as Cur/mPEG-PBLA micelles),and to evaluate the in vitro hepatoprotective activity against alcohol liver disease(ALD).METHODS Cur/mPEG-PBLA micelles were prepared with the dialysis method using methoxy-poly(ethylene glycol)-poly(β-benzyl-L-aspartate)(mPEG-PLGA)as the carrier.The appearance and microscopic morphology of Cur/mPEG-PBLA micelles were observed,and particle size,polydispersity index,Zeta potential,encapsulation efficiency and drug loading content were all detected.The in vitro release,pH stability,thermal stability,dilution stability,storage stability,plasma stability tests,and hemolysis experiments were all performed.The cell model of ALD was established with anhydrous ethanol intervention using human liver cancer cells and normal liver cells as objects,Cur reference solution as reference,to evaluate in vitro preventive and ameliorative effects of Cur/mPEG-PBLA micelles on ALD.RESULTS The prepared Cur/mPEG-PBLA micelles exhibited a pale-yellow milky light,with a spherical shape and uniform distribution.The average particle size was about 140 nm,and the polydispersity index was less than 0.3.Zeta potential was(-8.15±0.05)mV;the encapsulation efficiency was(73.26±3.16)%,and the drug loading content was(4.87±0.42)%.The cumulative release of Cur reference substance was close to 80%at 10 h;the cumulative release of Cur/mPEG-PBLA micelles at 8 h was 28.94%and only 48.25%at 48 h.pH stability and thermal stability of Cur/mPEG-PBLA micelles were better than those of Cur reference solution;Cur/mPEG-PBLA micelles showed good dilution stability,storage stability and plasma stability,and would not cause hemolysis.Cur reference solution and Cur/mPEG-PBLA micelles had varying degrees of in vitro preventive and ameliorative effects on ALD in two types of cells;after 48 h of application,the above effects of Cur/mPEG-PBLA micelles were significantly better than those of Cur reference solution at the same mass concentration(P＜0.05).CONCLUSIONS Cur/mPEG-PBLA micelles can improve pH stability and thermal stability of Cur,delayits degradation rate,and have better in vitro hepatoprotective activity against ALD.