中国生物防治学报2024，Vol.40Issue(2)：424-434,11.DOI:10.16409/j.cnki.2095-039x.2024.02.005
生防菌Y26的鉴定、发酵优化及其杀线虫活性测定
Identification,Fermentation Optimization and Determination of Nematicidal Activity of Biocontrol Bacterium Y26
摘要
Abstract
To control the root-knot nematode disease in cucumber,a bacterial strain Y26 was isolated from cucumber rhizosphere soil in the laboratory.Bio-activity of fermentation broth against root-knot nematodes was determined.The corrected mortality rate of the second-stage juveniles treated with fermentation broth for 24 hours was 95.12%,and the relative inhibition rate of eggs hatching reached 83.51%after 48 hours of fermentation broth treatment,so strain Y26 can be used as a bacterial control resource for root-knot nematodes disease.Strain Y26 was identified as Bacillus pumilus by morphological observation and molecular identification.The optimized conditions for fermentation of strain Y26 by Plackett-Burma design and response surface analysis were corn flour 2.14%,bean cake flour 3%,K2HPO40.57%,MgSO4·7H2O 0.21%,cultural time 12 hours,pH 7.2,inoculation amount 2%,cultural temperature 28℃,and the media amount was 50 mL/250 mL.After fermentation optimization,the spore concentration was increased by 25.69%,the corrected mortality rate of 10%fermentation broth increased from 78.03%to 93.32%before optimization.The results showed that the activity of optimized Y26 fermentation broth root-knot nematodes was significantly increased and could be developed and applicated as a biological nematicide.关键词
根结线虫/短小芽胞杆菌/鉴定/发酵条件优化Key words
root-knot nematode/Bacillus pumilus/identification/optimization of fermentation conditions分类
农业科技引用本文复制引用
梅凤珍,周明超,杨丹,李香风,孔令安,刘建斌,史凤玉..生防菌Y26的鉴定、发酵优化及其杀线虫活性测定[J].中国生物防治学报,2024,40(2):424-434,11.基金项目
河北省重点研发计划项目(21326502D) （21326502D）
植物病虫害生物学国家重点实验室开放基金(SKL0F202115) （SKL0F202115）
