中国临床药理学杂志2024，Vol.40Issue(9)：1267-1271,5.DOI:10.13699/j.cnki.1001-6821.2024.09.006
突触核蛋白γ对口腔鳞癌细胞迁移和侵袭作用的研究
Study on the effect of synaptic nuclear protein γ on migration and invasion of oral squamous cell carcinoma cells
摘要
Abstract
Objective Lentivirus-mediated interference with synaptic nuclear protein γ(SNCG)in human oral squamous cell carcinoma was established to study the role of SNCG in the migration and invasion of oral squamous cell carcinoma.Methods Oral cancer CAL27 cells were infected with LV-shNC and LV-shSNCG constructed by lentivirus vector,respective,and then selected with puromycin to obtain cell lines stably interfering with SNCG,which were named NC group and experimental group,respectively.Detect the expression of SNCG through real-time quantitative polymerase chain reaction(qRT-PCR)and Western blot experiments;Transwell and scratch experiments were used to detect changes in migration and invasion ability.Results Compared with the NC group,the experimental group showed an 80％reduction in SNCG mRNA expression(P＜0.01).The relative expression level of SNCG protein was also decreased in the experimental group compared to the NC group(P＜0.01).In the NC group and the experimental group,the migration area percentages at 36 hours were 0.54±0.06 and 0.40±0.02,respectively;and at 48 hours were 0.83±0.01 and 0.47±0.05,respectively.The experimental group showed decrease in migration area compared to the NC group,and these differences were statistically significant(P＜0.05,P＜0.001).Compared to the NC group,the migration and invasion cell numbers in the experimental group(98.00±13.49 and 88.00±5.72)were significantly reduced to(48.00±2.16 and 49.00±2.94),and these differences were statistically significant(P＜0.01,P＜0.001).Conclusion Interference of SNCG expression can significantly reduce the migration and invasion ability of oral squamous cell carcinoma cells.关键词
人突触核蛋白γ/口腔鳞癌/慢病毒载体/迁移/侵袭Key words
synaptic nuclear protein γ/oral squamous cell carcinoma/lentiviral vector/migration/invasion分类
医药卫生引用本文复制引用
任作东,庄赵为,赵娟,袁武梅,曾妍..突触核蛋白γ对口腔鳞癌细胞迁移和侵袭作用的研究[J].中国临床药理学杂志,2024,40(9):1267-1271,5.基金项目
国家自然科学基金资助项目(81560473) （81560473）
