中国实验动物学报2024，Vol.32Issue(3)：286-296,11.DOI:10.3969/j.issn.1005-4847.2024.03.002
抗阻运动对SAMP8小鼠骨骼肌lncRNA和mRNA基因表达谱的影响
Effects of resistance exercise on long non-coding RNA and mRNA expression profiles of skeletal muscle in senescence accelerated-prone 8 mice
摘要
Abstract
Objective To explore the potential regulatory mechanism of resistance exercise in senescence accelerated-prone 8 mice(SAMP8)by evaluating the effects of exercise on the expression of long non-coding RNA(lncRNA)and mRNA in quadriceps muscles by RNA-sequencing(RNA-seq)technology.Methods Twenty-eight-week-old male SAMP8 mice were divided into a model group(M group)and resistance-exercise group(R group)(n=6 mice per group).Another eight normally aging SAMR1 mice of the same age were used as the control group(C group).Mice in R group received 8 weeks of increasing weight climbing exercise training.Relative grip strength was measured every week and the rotarod test was performed every 2 weeks.Histological changes in the right quadriceps femoris were observed by hematoxylin-eosin staining and the left quadriceps was used for RNA-seq.Differentially expressed lncRNA and mRNA were screened and analyzed for enrichment by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses.Finally,key differentially expressed genes were analyzed by quantitative reverse transcription-polymerase chain reaction to verify the accuracy of the RNA-seq result.Results(1)Relative grip strength and rotarod test time were significantly decreased in M group compared with C group(P＜0.01),but were significantly increased after 8 weeks of Rgroup compared with M group(P＜0.01).(2)The cross-sectional area of the muscle fibers was significantly lower in M group compared with C group,as shown by HE staining(P＜0.01),while the cross-sectional area of the muscle fibers was significantly increased in the R group compared with M group(P＜0.01).(3)Differential expression analysis identified 182 upregulated and 218 downregulated lncRNA,and 454 upregulated and 289 downregulated mRNA between M group and R group.The KEGG pathways of lncRNA target genes that were differentially expressed between M group and R group were significantly enriched in intestinal immune network for IgA production,nuclear factor-kappa B signaling pathway,and inflammatory bowel disease.Conclusions(1)This study demonstrated that resistance exercise can improve skeletal muscle function in SAMP8 mice with sarcopenia.We identified lncRNA and mRNA that were differentially expressed as a result of resistance exercise,and which might be potential targets of sarcopenia therapy.(2)Furthermore,analyzing the biological functions of the target genes of the differentially expressed lncRNA and mRNA may further our understanding of the mechanism of resistance exercise for improving sarcopenia.关键词
长非编码RNA/SAMP8/肌少症/转录组/抗阻运动Key words
long non-coding RNA/SAMP8/sarcopenia/transcriptome/resistance exercise分类
生物科学引用本文复制引用
傅泽铤,李仲豪,李伦宇,刘洪政,丁海丽..抗阻运动对SAMP8小鼠骨骼肌lncRNA和mRNA基因表达谱的影响[J].中国实验动物学报,2024,32(3):286-296,11.基金项目
国家自然科学基金(81904318),四川省自然科学基金(2023NSFSC0545),四川省中央引导地方科技发展项目(2022ZYD0062),国家体育总局运动医学重点实验室(2018YFF0300904),运动医学四川省重点实验室(2021-A030),四川省科技创新创业苗子工程项目(2023JDRC0100). Funded by the National Natural Science Foundation of China(81904318),Sichuan Natural Science Foundation(2023NSFSC0545),Sichuan Province Central Government Guides Local Science and Technology Development Project(2022ZYD0062),the Key Laboratory of Sports Medicine of the General Administration of Sport of China(2018YFF0300904),Key Laboratory of Sports Medicine of Sichuan Province(2021-A030),Sichuan Province Science and Technology Innovation and Entrepreneurship Seedling Project(2023JDRC0100). （81904318）
