中国实验动物学报2024，Vol.32Issue(3)：317-328,12.DOI:10.3969/j.issn.1005-4847.2024.03.005
乙酸灌肠联合束缚夹尾应激诱导肝郁脾虚型腹泻型肠易激综合征大鼠模型的制备
Preparation of a rat model of diarrheal irritable bowel syndrome induced by an acetic acid enema combined with binding tail-clamping stress
摘要
Abstract
Objective To establish an ideal modeling method for diarrhea predominant irritable bowel syndrome(IBS-D)with anxiely and depression in rats,and to provide a basis for the clinical study of IBS-D.Methods 60 rats were used in this study.(1)At first,20 rats were randomly divided into blank,3%acetic acid enema,4%acetic acid enema,and 5%acetic acid enema groups.After the modeling and observation period,the diarrhea status and the degree of colon injury caused by different modeling concentrations were observed by diarrhea related index and colon histopathology.(2)After the optimal modeling concentration was assessed,40 rats were randomly divided into control(a),acetic acid enema(b),acetic acid+binding(c),and acetic acid+binding+tail clip(d)groups and correspondingly treated for 8 days.After the treatments,the general condition,diarrhea-related index,open field test(OFT)score,and colonic histopathology of rats were evaluated.Results(1)Compared with the blank group,the fecal trait score of 4%acetic acid enema group was increased on days 1 to 3 after intervention(P＜0.001),and gradually decreased on days 4 to 7 after intervention.After 1 week,there was no significant difference between the fecal trait score and that of the blank group(P＞0.05).Body weight was lower(P＜0.01),fecal water content was higher(P＜0.001).Compared with blank group,body weight of the 5%acetic acid enema group was decreased(P＜0.001),the fecal trait score and diarrhea index were increased(P＜0.01).No significant difference was found between 3%acetic acid enema and blank groups.The pathological colon tissue showed that,compared with the blank group,the mucosal structure of the 4%acetic acid enema group was complete with a small amount of inflammatory cell infiltration,and the pathological tissue score showed no significant difference(P＞0.05),whereas the 5%acetic acid enema had a medium to large amount of inflammatory cell infiltration,and the pathological tissue score was increased(P＜0.01).(2)Compared with group a,group b had lower body weight(P＜0.001),and higher fecal trait score,fecal water content and diarrhea index(P＜0.01).Compared with a and b groups,the body weight of c and d groups was lower(P＜0.001),the fecal traits score,fecal water content,and diarrhea index were increased(P＜0.01),and the colon running time was decreased(P＜0.01).Compared with group c,Fecal water content in group D was higher(P＜0.001).In the OFT score,compared with a and b groups,the OFT distance,standing times,and upright times in c and d groups were lower(P＜0.05).Compared with c,the OFT distance,standing times,and upright times in d group were lower(P＜0.05).The pathological tissue of colon showed that the mucosal structure of the four groups was complete,and there were different degrees of inflammatory cell infiltration.The pathological tissue scores of groups c and d were higher than those of groups a and b(P＜0.05).Conclusions The 4%acetic acid concentration is appropriate for IBS-D modeling.After superposition and binding,the IBS-D diarrhea and internal hypersensitivity characteristic state can be better simulated.After superposition of a tail clip,the IBS-D model of liver stagnation and spleen deficiency can be established successfully.关键词
腹泻型肠易激综合征/4%乙酸灌肠/慢性束缚/夹尾应激/造模Key words
diarrheal predominant irritable bowel syndrome/4%acetic acid enema/chronic bondage/tail-clamping stress/molding分类
生物学引用本文复制引用
赖碧玉,聂晶,佘畅,洪梦颖,李兴,何永嘉,陈瑶,李新伍,石佳,田子寒,李丹..乙酸灌肠联合束缚夹尾应激诱导肝郁脾虚型腹泻型肠易激综合征大鼠模型的制备[J].中国实验动物学报,2024,32(3):317-328,12.基金项目
湖南省自然科学基金项目(2021JJ70066),湖南省中医药科研计划项目(B2023108,2020143),国家级大学生创新训练项目(202110541024),湖南中医药大学2022 年度校级科研项目(2022XYLH088). Funded by Natural Science Foundation of Hunan Province(2021JJ70066),Traditional Chinese Medicine Research Project of Hunan Province(B2023108,2020143),National Innovative Training Program for College Students(202110541024),Hunan University of Chinese Medicine 2022 University-Level Scientific Research Project(2022XYLH088). （2021JJ70066）
