不同方法建立小鼠肝癌原位移植瘤模型差异性的探讨OA北大核心CSTPCD
Different transplantation models of hepatocellular carcinoma in mice
目的 比较肿瘤细胞注射和肿瘤组织块移植方法建立小鼠肝癌原位模型的差异,为小鼠肝癌原位模型的建立提供技术参考.方法 取健康雄性KM小鼠,随机分为 4 组:A组注射小鼠肝癌H22 细胞;B组注射腹水型肝癌H22 细胞;C组采用小鼠肝癌组织块移植法;D组肝部注射生理盐水作为假手术组.定期观察各组小鼠活动情况、体重变化.记录 4 组小鼠的生存时间.观察各组小鼠肝部成瘤状况、肿瘤程度、与腹腔脏器粘连程度和转移情况,并进行 B 超成像,检测血清中甲胎蛋白(alpha-fetoprotein,AFP)、异常凝血酶原(des-gamma-carboxyprothrombin,DCP)的浓度及苏木素-伊红(HE)染色观察肝组织病理学变化.结果 A组、B组、C组小鼠造模操作时间分别为(3.36±0.44)min、(3.30±0.41)min、(5.68±0.65)min.建模第25 天,A组、B组、C组小鼠成瘤率均 100.0%,A组腹腔重度粘连率为 40.0%,B组腹腔重度粘连率为 60.0%,C组与D组均未出现重度腹腔粘连.A组、B组、C组小鼠腹水出现率分别为 40.0%、100.0%、0.0%;腹壁瘤出现率分别为 30.0%、60.0%、0.0%.B组小鼠还存在肝转移情况(40.0%).B超成像、血清AFP 和DCP 水平检测、组织病理学检查结果显示,与D组小鼠相比,A组、B组、C组小鼠肝边缘均不光滑,回声欠均匀,可见稍低回声包块;保持AFP、DCP高分泌;存在大量炎症细胞与肿瘤细胞.结论 在第 25 天时,3 种方法均可以建立肝癌原位移植模型,其中,相较于肝癌组织块移植,肿瘤细胞注射具有操作简单、肝内存在多个转移结节的特点.而肝癌组织块移植相较于肿瘤细胞注射,则具有对腹腔等脏器影响较小等特点.
Objective To explore the otherness of orthotopic injection of cell suspensions and transplantation of tumor tissue blocks to establish orthotopic implantation models of hepatocellular carcinoma in mice,and to provide a technical reference for the establishment of an orthotopic implantation model.Methods Healthy KM mice were divided into four groups:group A,direct injection of H22 cells;group B,direct injection of H22 ascitic cells;group C,transplantation of tissues;and group D,direct injection of saline.Activity and weight changes were observed regularly in each group and survival times were recorded.Liver tumor formation,tumor size,abdominal organ adhesion degree,and metastasis were observed in all groups.B-ultrasound imaging was performed,concentrations of alpha fetoprotein(AFP)and abnormal prothrombin(DCP)were detected,and liver histopathological changes were detected by hematoxylin and eosin staining.Results Mice molding operation time in groups A,B,and C were(3.36±0.44)min,(3.30±0.41)min,and(5.68±0.65)min,respectively.After modeling for 25 days,the rates of model formation in groups A,B,and C were all 100.0%.Severe abdominal adhesions occurred in 40.0%of mice in group A and 60.0%in group B,but in no mice in group C or D.Ascites occurred in 40.0%,100.0%,and 0.0%and abdominal wall tumors in 30.0%,60.0%,and 0.0%of mice in groups A,B,and C,respectively,while 40.0%of mice in group B also had liver metastasis.B-ultrasound imaging,detection of serum AFP and DCP levels,and histopathological result showed smooth liver margins,uneven echo and slightly lower echo mass,maintained high AFP and DCP secretion,and large numbers of inflammatory cells and tumor cells in mice in groups A,B,and C.Conclusions At day 25,all three methods can thus be used to establish orthotopic transplantation models of HCC.Among these,inj ection of cell suspensions demonstrated the advantage of simplicity in operation and the presence of multiple metastatic nodules within the liver,compared to transplantation of tumor tissue.Conversely,transplantation of tumor tissue showed the advantage of causing less impact on the abdomen and other organs when compared to inj ection of cell suspensions.
潘蕊;喻锟;张海亮;郑永仁;赵笑雨;唐钧泽;吴健铭;程欣
云南中医药大学中药学院,昆明 650500||云南省南药可持续利用研究重点实验室,昆明 650500||云南省傣医药与彝医药重点实验室,昆明 650500云南中医药大学中药学院,昆明 650500云南中医药大学中药学院,昆明 650500||云南省傣医药与彝医药重点实验室,昆明 650500云南中医药大学科学技术处,昆明 650500云南中医药大学中药学院,昆明 650500||云南省高校外用给药系统与制剂技术研究重点实验室,昆明 650500||云南省傣医药与彝医药重点实验室,昆明 650500
生物学
肝癌原位移植动物模型B超成像
liver cancerorthotopic transplantationanimal modelB-ultrasound imaging
《中国实验动物学报》 2024 (003)
329-336 / 8
云南省科技厅-云南中医药大学应用基础研究联合专项重点项目(202001AZ070001-008),云南省科技厅生物医药重大科技专项(202002AA100007),云南省科技人才平台计划(202105AG070012),云南省傣医药与彝医药重点实验室开放课题(202210ZD2207,202210SS2205,202210SS2206),国家中医药管理局"十二五"重点学科-傣药学. Funded by Yunnan Provincial Department of Science and Technology-Yunnan University of Chinese Medicine Applied Basic Research Joint Project(202001AZ070001-008),Major Scientific and Technological Projects for Biomedical Industry of Yunnan Provincial Science and Technology Department(202002AA100007),Yunnan Province Science and Technology Talent Platform Plan(202105AG070012),Yunnan Province Key Laboratory of Dai Medicine and Yi Medicine Open Project(202210ZD2207,202210SS2205,202210SS2206),Key Discipline of Twelfth Five-Year Plan of State Administration of Traditional Chinese Medicine-Dai Pharmacy.
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