中国药理学与毒理学杂志2024，Vol.38Issue(5)：369-374,6.DOI:10.3867/j.issn.1000-3002.2024.05.006
天麻素可能通过抑制自噬信号改善大鼠肾性高血压
Gastrodin improves renal hypertension in rats possibly by inhibiting autophagy signals
摘要
Abstract
OBJECTIVE To explore the mechanism by which gastrodin improves renal hyperten-sion in rats.METHODS A rat model of renal hypertension was established by ligating the renal artery.seventy-five rats were randomly divided into 5 groups(n=15):control group(sham operation group),model group,model+ramipril 1 mg·kg-1,model+gastrodin 100 and 200 mg·kg-1.The systolic blood pressure of the tail artery after modeling>18.12 kPa was regarded as the success of modeling.After the model was established,rats in the model+ramipril group were ig given ramipril 1 mg·kg-1 while the model+gastrodin group was ig given gastrodin 100 and 200 mg·kg-1 respectively for 4 weeks.Colori-metric assay kit was used to detect the serum superoxide dismutase(SOD)activity and malondialde-hyde(MDA)content in rats.ELISA was used to detect serum angiotensin-2(Ang-2)and aldosterone(ALD)contents,as well as serum and thoracic aortic tissue interleukin-1β(IL-1β),IL-6 and tumor necrosis factor α(TNF-α)content.HE staining was performed to detect pathological changes in thoracic aorta tissue of rats.The expressions of autophagy protein miceotubule associated protein light chain 3(LC3)-Ⅱand LC3-Ⅰwere detected by Western blotting.RESULTS The systolic pressure of the tail artery of rats after modeling exceeded 18.12 kPa,indicating that the modeling was successful.Compared with the control group,the contens of Ang-2(P<0.01)and ALD(P<0.01)in the model group were significantly increased,the activity of SOD(P<0.01)in serum and thoracic aorta tissue was significantly decreased,the content of MDA(P<0.01)was significantly increased,the contents of IL-1β,IL-6 and TNF-α in serum and thoracic aorta tissue were significantly decreased(P<0.01)and the ratio of LC-32/LC-31 in thoracic aorta tissue was significantly increased(P<0.01).Compared with the model group,gastrodin significantly increased the systolic pressure of the tail artery(P<0.01),the contents of Ang-2(P<0.01)and ALD(P<0.01)and the activity of SOD(P<0.01)in serum,as well as decreased the contents of MDA,IL-1β,IL-6 and TNF-α(P<0.01)in serum and thoracic aorta tissue.Meanwhile,gastrodin signifi-cantly decreased the ratio of LC3-Ⅱ/LC3-Ⅰ in rat thoracic aorta(P<0.01).CONCLUSION Gastrodin can improve the blood pressure of renal hypertensive rats,and the mechanism is possibly related to the reduction of autophagy protein LC3-Ⅱ/LC3-Ⅰratio.关键词
天麻素/肾性高血压/主动脉/自噬Key words
gastrodin/renal hypertension/thoracic aorta/autophagy分类
医药卫生引用本文复制引用
司丽芳,赵天智,宋毓民,李梦云,李翔,王飞耀..天麻素可能通过抑制自噬信号改善大鼠肾性高血压[J].中国药理学与毒理学杂志,2024,38(5):369-374,6.基金项目
国家自然科学基金(31401209) （31401209）
河南科技大学青年科学基金(2019Qn024) （2019Qn024）
河南科技大学重大校企合作项目(2020XQ39) （2020XQ39）
动物营养调控与健康安徽省重点实验室基金(APKLANRH202004) National Natural Science Foundation of China(31401209) （APKLANRH202004）
Youth Science Foundation of Henan Uni-versity of Science and Technology(2019Qn024) （2019Qn024）
Major School Enterprise Cooperation of Henan University of Science and Technology Project(2020XQ39) （2020XQ39）
and Animal Nutrition Regulation and Health Anhui Provincial Key Laboratory Fund Project(APKLANRH202004) （APKLANRH202004）
