中国实验动物学报2024，Vol.32Issue(3)：362-368,7.DOI:10.3969/j.issn.1005-4847.2024.03.010
猫棒束孢联合环磷酰胺对H22荷瘤小鼠的治疗作用
Therapeutic effects of Isaria felina combined with cyclophosphamide in hepatoma H22 tumor-bearing mice
摘要
Abstract
Objective To investigate the therapeutic effects of Isaria felina derived from Cordyceps sinensis combined with cyclophosphamide(CTX)in hepatoma H22 tumor-bearing mice.Methods An H22 tumor-bearing mouse model was established and mice were divided randomly into a normal control group(NC group,distilled water),model control group(MC group,distilled water),positive control group(CTX group,25 mg/kg),Isaria felina group(IF group,400 mg/kg),and combined administration group(IF+CTX group,IF 400 mg/kg+CTX 25 mg/kg),with 5 mice in each group.Distilled water and IF were administered by gavage,and CTX was administered by intraperitoneal injection.The administration cycle was 10 days.At the end of the experiment,the mean tumor volume and weight,tumor inhibition rate,q value,and immune organ index were calculated,and routine blood indexes and cytokine levels were determined.Histopathological changes in tumor tissues were observed by HE staining.Results The tumor volume and mass were significantly lower in mice in each treatment group compared with those in mice in the MC group(P＜0.05).The tumor inhibition rates in the CTX,IF,and IF+CTX groups were 49.3%,34.2%,and 72.8%,respectively,and the q value was 1.09.The numbers of white blood cells,Lymph,and platelets were significantly higher in the IF+CTX group than in the CTX group(P＜0.05).The spleen index was significantly higher in the MC group compared with that in the NC group,and significantly lower in the IF+CTX group compared with that in the MC group(P＜0.05).Serum interferon-γ levels were significantly lower in the MC group than in the NC group,and were significantly higher in the IF and IF+CTX groups compared with those in the MC and CTX groups(P＜0.05).Pathologically,tumor cells in the MC group grew well and were numerous and closely arranged,while cells in the CTX,IF,and IF+CTX groups were arranged loosely,with focal necrosis and nuclear pyknosis of necrotic cells in many places.Conclusions The combination of IF and CTX has an additive anti-tumor effect on H22 tumor-bearing mice,which can alleviate immunosuppression and have an immunomodulatory function.关键词
猫棒束孢/H22荷瘤小鼠/联合给药/免疫调节Key words
Isaria felina/H22 tumor-bearing mice/combined administration/immunomodulation分类
生物科学引用本文复制引用
史晓伟,杨喜花,陈静静,余国彦,张艺尹,陈丽霞,赵莉莉,杨永明,王靖,阎磊..猫棒束孢联合环磷酰胺对H22荷瘤小鼠的治疗作用[J].中国实验动物学报,2024,32(3):362-368,7.基金项目
山西省卫生健康委员会科研课题计划(2021072). Funded by the Health Commission of Shanxi Provincial Research Project(2021072). （2021072）
