中国实验动物学报2024，Vol.32Issue(3)：369-377,9.DOI:10.3969/j.issn.1005-4847.2024.03.011
小肠过表达Ski蛋白对扭体小鼠的抑炎镇痛作用
Anti-inflammatory and analgesic effects of small intestine Ski-overexpression on acetic acid-induced writhing mice
摘要
Abstract
Objective To study the anti-inflammatory and analgesic effects of Ski protein overexpression on writing in mice induced by acetic acid.Methods Eight-week-old male ICR mice were administered 0.7%acetic acid solution(0.1 mL/10 g)to induce a writhing reaction.The mice were divided into sham,acetic acid,acetic acid+ibuprofen,acetic acid+ad-EGFP,acetic acid+ad-ski-1,acetic acid+ad-ski-2,and acetic acid+sulfasalazine groups(n=10 mice per group).The time to the first appearance of twisting and the number of twists within 15 min were recorded.Small intestine tissues were removed to identify the effect of adenovirus transfection and to detect protein expression levels of pro-inflammatory factors and pain biomarkers and protein expression of nuclear factor(NF)-κB p65 and its binding with Ski protein.Results Ski protein was successfully overexpressed in small intestine after intraperitoneal injection of Ad-ski adenovirus.Overexpressed Ski protein delayed the start and decreased the frequency of writhing,comparable to ibuprofen(P＞0.05).Groups in which ski protein was overexpressed showed significantly inhibited protein expression of pro-inflammatory factors and pain biomarkers compared with the acetic acid group(P＜0.05).Moreover,NF-κB p65 formed complexes with Ski.Conclusions Overexpression of Ski protein has anti-inflammatory and analgesic effects on acetic acid-induced inflammatory pain by inhibiting the expression of inflammatory factors and pain biomarkers,via regulation of the NF-κB signaling pathway.关键词
Ski/扭体实验/白细胞介素-1β/肿瘤坏死因子-α/神经生长因子/酪氨酸蛋白激酶A/小鼠Key words
Ski/writhing test/IL-1β/TNF-α/nerve growth factor/tyrosine protein kinase A/mice分类
生物学引用本文复制引用
熊翱,魏小雨,彭艳,杨明,杨钱冬,罗飞..小肠过表达Ski蛋白对扭体小鼠的抑炎镇痛作用[J].中国实验动物学报,2024,32(3):369-377,9.基金项目
重庆市研究生(博士)科研创新课题(CYB23292),陆军军医大学优秀人才库重点扶持对象(XZ-2019-505-021). Funded by the Postgraduate(Doctor)Research Innovation Project of Chongqing(CYB23292),Key Supporters of the Excellent Talent Pool of the Army Medical University(XZ-2019-505-021). （博士）
