中国实验动物学报2024，Vol.32Issue(3)：378-384,7.DOI:10.3969/j.issn.1005-4847.2024.03.012
奥拉帕尼诱导乳腺癌MCF-7细胞衰老作用及其机制
Effect and mechanism of olaparib on senescence of MCF-7 breast cancer cells
摘要
Abstract
Objective To study the cellular senescence and molecular mechanism of olaparib in MCF-7 breast cancer cells.Methods The effects of olaparib on the proliferation and migration of MCF-7 cells were detected dynamically by real-time cell analysis(RTCA)technology.The effects of olaparib on the Senescence was detected by using the senescence-associated β-galactosidase(SA-β-gal).Quantitative polymerase chain reaction was used to analyze the effects of olaparib on the expression levels of genes encoding the senescence-associated factors p16,p21,C/EBP homologous protein,interleukin(IL)-6,IL-8,plasminogen activator inhibitor 1,phosphatase and tensin homolog deleted on chromosome 10,p27,retinoblastoma gene,Ki67,and E2F1.The effects of olaparib on the expression levels of the senescence-associated proteins p21,γH2AX,pRB,cyclin D1,insulin-like growth factor binding protein 3,and Ki67 were analyzed by Western Blot.Results Olaparib inhibited the proliferation and migration and induced the senescence of MCF-7 cells.Long-term(96 h)treatment with olaparib significantly up-regulated the gene expression levels of p16,p21,p27,C/EBP homologous protein,IL-6,IL-8,plasminogen activator inhibitor 1,phosphatase and tensin homolog deleted on chromosome 10,and retinoblastoma protein(P＜0.01)and significantly down-regulated the gene expression levels of Ki67 and E2F1(P＜0.01)in MCF-7 cells.Olaparib significantly increased protein expression levels of p21,γH2AX,and insulin-like growth factor binding protein 3 in MCF-7 cells(P＜0.01,P＜0.01,P＜0.05)and significantly decreased cyclin D1,pRB,and Ki67 levels(P＜0.05,P＜0.01,P＜0.05).Conclusions Olaparib can inhibit proliferation and migration and induce senescence in MCF-7 breast cancer cells.关键词
奥拉帕尼/乳腺癌/衰老Key words
olaparib/breast cancer/senescence分类
生物科学引用本文复制引用
王大伟,郭晶,边继春,王莎莎,卢美超,张岱州,贾玉萍..奥拉帕尼诱导乳腺癌MCF-7细胞衰老作用及其机制[J].中国实验动物学报,2024,32(3):378-384,7.基金项目
济南市"高校20 条"资金项目(2020GXRC043),中央引导地方科技发展专项资金项目(YDZX2021023),复旦大学分子病毒学重点实验室开放课题项目(FDMV-2021005). Funded by Jinan's"University 20"Project(2020GXRC043),the Central Government's Special Project for Guiding Local Science and Technology Development(YDZX2021023),the Open Project of the Key Laboratory of Molecular Virology of Fudan University(FDMV-2021005). （2020GXRC043）
