心脑血管病防治2024，Vol.24Issue(4)：15-18,23,5.DOI:10.3969/j.issn.1009-816x.2024.04.004
细胞外基质金属蛋白酶诱导子突变的血管平滑肌细胞的构建及其功能研究
Construction and functional study of vascular smooth muscle cells with extracellular matrix metalloproteinase inducer mutation
摘要
Abstract
Objective To explore the effects of extracellular matrix metalloproteinase inducer(EMMPRIN)on the proliferation,migration and inflammatory response of vascular smooth muscle cells(VSMCs).Methods CRISPR/Cas9 technology was used to construct EMMPRIN c.889C>A-point mutation VSMC line.Real-time qPCR and Western blot were performed to detect the expression level of EMMPRIN.Cell proliferation activity was measured using the CCK-8 assay,while cell migration capability was evaluated using the Transwell assay.Secretion of interleukin-6(IL-6),monocyte chemoattractant protein-1(MCP-1),and interleukin-1β(IL-1β)was detected using enzyme-linked immunosorbent assay(ELISA).Results Compared to the wild-type(WT)cells,the expression level of EMMPRIN did not exhibit significant changes in c.889C>A mutant cells(t=0.732,P>0.05).There was no statistically significant difference in cell proliferation activity(P>0.05),while cell migration capacity significantly decreased(t=5.121,P<0.05).Secretion of inflammatory cytokines IL-6,MCP-1 and IL-1β induced by TGF-β decreased markedly in the c.889C>A mutant cells(t=9.371,5.690,6.192;P<0.05).Conclusion The EMMPRIN c.889C>A mutation does not affect the expression of EMMPRIN or VSMCs proliferation.However,it can supress the migration of VSMCs and the secretion of inflammatory cytokines.关键词
细胞外基质金属蛋白酶诱导子/血管平滑肌细胞/增殖/迁移Key words
Extracellular matrix metalloproteinase inducer/Vascular smooth muscle cells/Proliferation/Migration引用本文复制引用
张云燕,林威钢,徐程,张芸,方崇峰..细胞外基质金属蛋白酶诱导子突变的血管平滑肌细胞的构建及其功能研究[J].心脑血管病防治,2024,24(4):15-18,23,5.基金项目
浙江省台州市科技计划项目(1801ky13) （1801ky13）
