细胞外基质金属蛋白酶诱导子突变的血管平滑肌细胞的构建及其功能研究OACSTPCD
Construction and functional study of vascular smooth muscle cells with extracellular matrix metalloproteinase inducer mutation
目的 探讨细胞外基质金属蛋白酶诱导子(EMMPRIN)c.889C>A点突变对血管平滑肌细胞增殖、迁移及炎症反应等功能的影响.方法 利用CRISPR/Cas9 技术构建EMMPRIN c.889C>A点突变血管平滑肌细胞系;Real-time qPCR和Western blot实验检测EMMPRIN的表达水平;CCK-8 实验检测细胞增殖活性;Transwell实验检测细胞迁移能力;酶联免疫吸附法检测白细胞介素6(IL-6)、巨噬细胞…查看全部>>
Objective To explore the effects of extracellular matrix metalloproteinase inducer(EMMPRIN)on the proliferation,migration and inflammatory response of vascular smooth muscle cells(VSMCs).Methods CRISPR/Cas9 technology was used to construct EMMPRIN c.889C>A-point mutation VSMC line.Real-time qPCR and Western blot were performed to detect the expression level of EMMPRIN.Cell proliferation activity was measured using the CCK-8 assay,while cell migration capa…查看全部>>
张云燕;林威钢;徐程;张芸;方崇峰
317000 临海,温州医科大学附属台州医院心血管内科317000 临海,温州医科大学附属台州医院整形科317000 临海,温州医科大学附属台州医院心血管内科317000 临海,温州医科大学附属台州医院心血管内科317000 临海,温州医科大学附属台州医院心血管内科
细胞外基质金属蛋白酶诱导子血管平滑肌细胞增殖迁移
Extracellular matrix metalloproteinase inducerVascular smooth muscle cellsProliferationMigration
《心脑血管病防治》 2024 (4)
15-18,23,5
浙江省台州市科技计划项目(1801ky13)
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