中国蔬菜Issue(5)：51-58,8.DOI:10.19928/j.cnki.1000-6346.2024.0027
基于非靶向代谢组学对青花菜花球表面蜡粉代谢物的差异分析
Differential Analysis of Metabolites in Wax Powder on Surface of Broccoli Florets Based on Non-targeted Metabolomics
摘要
Abstract
In order to reveal the composition difference of the wax powder metabolites on the surface of the broccoli inflorescence,taking the wax powder deficient mutant and the wild type of broccoli as test materials,based on the non-targeted metabolomics of gas chromatography tandem time-of-flight mass spectrometry(GC-TOF-MS),the main metabolic pathways and their products of the broccoli wax powder deficient mutant and the wild type were deeply analyzed,and the significantly different metabolites were screened by using univariate and multivariate variable statistical analysis and database annotation.The results showed that a total of 24 significantly different metabolites were detected in the wax powder of broccoli buds,including 14 kinds of amino acids,4 kinds of organic acids,1 kind of fatty acid,1 kind of amine,1 kind of sugar,and 3 kinds of other secondary metabolites.Among them,there were 18 kinds of down-regulated,including 13 kinds of amino acids.Through comprehensive enrichment analysis and topological analysis,11 important metabolic pathways with prominent significance were obtained,among which the most significantly different metabolic pathway was the biosynthesis of amino acids,followed by the biosynthesis of secondary metabolites;and the most important one was tryptophan metabolism.Therefore,it is speculated that the lack of broccoli wax powder can regulate the content such as amino acids and organic acids and other secondary metabolites in the broccoli buds by regulating the pathways such as tryptophan metabolism,the biosynthesis of secondary metabolites,and the biosynthesis of amino acids,then regulate the growth of the inflorescence,the nutritional quality,and the cold resistance.关键词
青花菜/花蕾/蜡粉/气相色谱-质谱/代谢组学Key words
broccoli/bud/wax powder/gas chromatography tandem time-of-flight mass spectrometry(GC-TOF-MS)/metabolomics引用本文复制引用
陶美奇,潘永飞,山溪,戴忠良,张振超..基于非靶向代谢组学对青花菜花球表面蜡粉代谢物的差异分析[J].中国蔬菜,2024,(5):51-58,8.基金项目
镇江市农业科学院青年基金项目(QNJJ2021003),句容市科技项目(ZA32209),江苏省种业振兴揭榜挂帅项目(JBGS[2021]077),江苏省重点研发计划项目(BE2021376) （QNJJ2021003）
