中国蔬菜Issue(5)：69-74,6.DOI:10.19928/j.cnki.1000-6346.2024.0024
河北保定地区黄瓜花叶病毒与马铃薯Y病毒复合侵染叶用莴苣分子鉴定
Molecular Identification of Co-infection of Cucumber mosaic virus and Potato virus Y on Lettuce in Baoding,Hebei
摘要
Abstract
The suspected lettuce virus disease stain collected from Baoding City,Hebei Province was molecular biology identified by RT-PCR method,and the phylogenetic tree was constructed.The results showed that specific primers CMV and PVY amplified single bands with sizes of 260 bp and 420 bp,respectively.Through nucleotide sequence Blast alignment and phylogenetic tree analysis,it was found that the lettuce virus isolate from Baoding City,Hebei Province was clustered on the same branch as the Indian tomato CMV isolate(MN514839.1)and the Indian potato PVY isolate(JX945850.1),with homology of 95%and 92%,respectively.The research first detected that the lettuce virus disease was caused by the combined infection of CMV and PVY in Baoding City,Hebei Province.关键词
叶用莴苣/黄瓜花叶病毒/马铃薯Y病毒/复合侵染Key words
lettuce/Cucumber mosaic virus(CMV)/Potato virus Y(PVY)/complex infection引用本文复制引用
陈利达,李畅,李敬蕊,吕桂云,吴晓蕾,宫彬彬,高洪波..河北保定地区黄瓜花叶病毒与马铃薯Y病毒复合侵染叶用莴苣分子鉴定[J].中国蔬菜,2024,(5):69-74,6.基金项目
河北省现代农业产业技术体系设施蔬菜创新团队项目(HBCT2021030213),河北省重点研发计划项目(21326903D),承德市科技计划项目(202305B044) （HBCT2021030213）
