中国蔬菜Issue(5)：75-83,9.DOI:10.19928/j.cnki.1000-6346.2024.0017
芥蓝游离小孢子培养技术优化及其在育种中的应用
Optimization and Application in Breeding of Isolated Microspore Culture Technology in Chinese Kale
摘要
Abstract
Taking 16 Chinese kale F1 hybrids as donor material,this paper compared and analyzed the effects of optimized buds disinfection,isolated microspore release and filtering on culture of Chinese kale free microspore.The results indicated that the 16 tested Chinese kale donor materials all had embryogenesis capability,and obtained regenerated plants of different types.Thus,a larger-scale and large quantities doubled haploid(DH)of Chinese kale were obtained.Through comprehensive evaluation and screening in the field,these DH lines by isolated microspore culture technology of Chinese kale were used in breeding,and 3 new Chinese kale F1 hybrids'20M4 Jielan''21M2 Jielan''22M11 Jielan'with different maturity were bred.关键词
芥蓝/游离小孢子/单核靠边期/双单倍体Key words
Chinese kale/isolated microspore/late uninucleate stage/doubled haploid(DH)引用本文复制引用
张德双,李佩荣,王姣,卢桂香,赵泓,辛晓云,张凤兰,余阳俊,于拴仓,苏同兵,赵岫云,汪维红..芥蓝游离小孢子培养技术优化及其在育种中的应用[J].中国蔬菜,2024,(5):75-83,9.基金项目
"十四五"国家重点研发计划项目(2022YFD1200805),国家大宗蔬菜产业技术体系项目(CARS-23-A-05),北京蔬菜种质库资源繁育、优选与创新项目(KJCX20230126),北京市设施蔬菜创新团队项目(BAIC01-2023) （2022YFD1200805）
