栽培模式对龙牙百合种植地土壤微生物多样性的影响OACSTPCD

In order to reduce the use of pesticides in the cultivation process of Lilium brownii var.Viridulum and improve its yield and quality,a blank field,plastic film coverage,and one-time fertilization planting(PM+OTF)model treatment and traditional open field cultivation(OFC)model treatment were set up to compare the diversity of microbiota and fungi in the soil of different treatments,and analyze the impact of planting mode changes on the underground growth microbial environ-ment of Lilium brownii var.viridulum.The results showed that the richness of soil fungal communities in the PM+OTF mo-del was lower than that in the OFC model,but there was no significant difference in community diversity.There was no sig-nificant difference in bacterial richness and diversity between the two cultivation modes of soil.The PCA principal compo-nent analysis showed that the soil microbial community structure composition of the PM+OTF model was closer to that of the blank field.The analysis of soil fungal and bacterial community composition shows that there is no significant difference in microbial diversity at the phylum level between the two cultivation modes.However,there were significant differences(P<0.05)in bacterial genera such as Aquisphaera,Bacillus,and Acidothermus,while there were significant differences(P<0.05)in fungal genera such as Clavaria,Talaromyces,Mortierella,Ustilaginoidea,Fusarium,and Penicillium.In terms of yield,compared with the OFC model,the PM+OTF model increased yield by 10.5%,improved yield by 32%,reduced bulb diseases by 15.2%,and significantly reduced leaf diseases.Overall analysis shows that mulching with plastic film and one-time fertilization planting have the potential to control and reduce the occurrence of diseases in Lilium brownii var.viridulum.