中国烟草科学2024，Vol.45Issue(2)：90-98,9.DOI:10.13496/j.issn.1007-5119.2024.02.011
普通烟草外向整流Shaker K+通道NtSKOR1的组织表达分析
Tissue Expression Analysis of Outwardly-rectifying Shaker K+Channel Gene NtSKOR1 in Nicotiana tabacum
摘要
Abstract
The Stelar K+outward rectifier channel(SKOR)is a type of outwardly-rectifying Shaker K+channel located in the plasma membrane of column cells in plant roots.This study aimed to investigate the promoter activity and expression of the NtSKOR1 gene at different developmental stages.For this purpose,a 2439 bp promoter segment upstream of the start codon of NtSKOR1 was cloned and ProNtSKOR1∷GUS transgenic lines were generated.GUS staining and RT-qPCR assays were conducted to assess the activity and expression,respectively.The prediction of cis-acting elements revealed the presence of several elements related to light,stress,and hormone responses.During the full seedling stages,no evident GUS activity was observed.At the small cross-shaped stage,GUS activity was detected in the stem apex meristem tissues and the veins of two true leaves.During the root spreading stage,GUS activity was mainly observed in the vascular tissues of the stem,and weak activity began to appear in the vascular tissues of root and gradually increased as the tobacco plants grow.At the most flowering stage,the activity was primarily detected in the vascular tissues of the roots,stems,and upper leaves of tobacco.These findings were consistent with the results of RT-qPCR analysis.In conclusion,the expression of NtSKOR1 initiates at the small cross-shaped stage in tobacco plants and is mainly localized in the vascular tissues of the roots,stems,and leaves.Furthermore,at the most flowering stage,NtSKOR1 expression is significantly higher in the upper leaves,which have strong photosynthetic capacity,compared to the lower leaves with weak photosynthetic capacity.This suggests that NtSKOR1 may play a crucial role in the transport of K+ions and photosynthetic products in tobacco plants.关键词
NtSKOR1/ShakerK+通道/GUS活性/烟草/基因表达Key words
NtSKOR1/Shaker K+channel/GUS staining/tobacco/gene expression分类
农业科技引用本文复制引用
农童嘉,王倩,戴培刚,徐方正,袁佳苹,向海英,王学瑛,袁光,OLUWASEYI Setonji Hunpatin,崔萌萌,宁扬..普通烟草外向整流Shaker K+通道NtSKOR1的组织表达分析[J].中国烟草科学,2024,45(2):90-98,9.基金项目
国家自然基金面上项目(32170387) （32170387）
中国烟草总公司烟草基因组计划与生物育种重大科技项目[110202101035(JY-12)] （JY-12）
安徽省烟草公司科技项目(20220551002003008) （20220551002003008）
