吴门医派验方通窍止痛方对偏头痛模型大鼠的改善作用及机制研究OA

Objective:To investigate the effects of Wumen proved prescription Tongqiao Zhitong Formula(TZF)on migraine model rat serum nitric oxide(NO),calcitonin gene-related peptide(CGRP),endothelin-1(ET-1),neurotransmitter dopamine(DA),norepinephrine(NE),and serotonin(5-HT),and its mechanism.Methods:SD rats were randomly assigned into blank group,model group,flunarizine group,and TZF group,with 8 rats in each group,and the rats were gavaged once a day for 7 days.Thirty min after the last gavage,the migraine rat model was prepared by subcutaneous injection of glyceryl trinitrate(GTN)into the neck at the dose of 10 mg/kg in all groups except the blank group.The number of scratching of the head within 2 h(30 min interval)was observed,and the behavioral changes were recorded.The levels of NO,CGRP and ET-1 in serum and DA,NE and 5-HT in brain tissue were detected by ELISA 4 h after modeling.Results:Compared with the blank group,the number of head scratches in each time period of rats in the model group significantly increased(P<0.01),the levels of NO and CGRP in serum significantly increased(P<0.01),and the level of ET-1 was significantly decreased(P<0.01).The levels of DA,NE and 5-HT in brain tissues were significantly reduced(P<0.01).Compared with the model group,in flunarizine group and TZF group,the number of head scratches in each time period was significantly reduced(P<0.05 or P<0.01),the levels of NO and CGRP in serum decreased(P<0.05 or P<0.01),the level of ET-1 were significantly increased(P<0.05 or P<0.01),and the levels of DA,NE and 5-HT in brain tissue were significantly increased(P<0.01 or P<0.05).Conclusion:The improvement effect of Tongqiao Zhitong Formula on migraine rat model may be related to the regulation of serum NO,CGRP,ET-1 and the content of brain tissue neurotransmitters DA,NE and 5-HT.