基于蛋白质组学技术探讨通关藤注射液治疗小鼠肺癌的作用机制OA

Objective:To study the effect of Tongguanteng Injection on the growth and protein expression of lung cancer tissue in mice based on proteomics technology,and explore its mechanism.Methods:Forty BALB/c male mice were selected and a subcutaneous lung cancer transplantation model was established by inoculating Lewis lung cancer tumor strain under the armpit.They were randomly divided into a model group,a cisplatin group,and high-dose and low-dose groups of Tongguanteng Injection,with 10 mice in each group.The tumor growth of mice was recorded before and after administration.On the 21st day of administration,the mice were euthanized,blood was collected from the eyeballs,tumor tissue was taken,and the volume and mass of the tumor were measured.Tumor growth was evaluated by measuring tumor size and calculating tumor inhibition rate.Serum samples were taken,and quantitative proteomics was used to analyze the influence of protein expression differences in serum,and the signal pathways involved in the differential proteins were analyzed.Results:Compared with the model group,the tumor tissue in the high-dose and low-dose groups of Tongguanteng Injection was significantly reduced(P<0.01).A total of 6 272 proteins were quantitatively obtained,and 176 different proteins(59 up-regulated and 117 down-regulated)were detected between the administration group and the model group.The functions of these differential proteins mainly included gene expression,cellular biosynthesis,DNA template transcription,etc.Analysis of signal pathways enriched with differential proteins revealed that the PI3K/Akt signaling pathway,HIF-1 signaling pathway,ErbB signaling pathway,myocardial contraction,shigellosis,systemic lupus erythematosus,insulin signaling pathway,microbial metabolism in different environments,cAMP signaling pathway,messenger RNA monitoring pathway,etc.might be closely related to the proliferation and survival of tumor cells.Conclusion:The mechanism of the inhibitory effect of Tongguanteng Injection on tumor growth in lung cancer mice may be closely related to the PI3K/Akt signaling pathway,HIF-1 signaling pathway,ErbB signaling pathway,and other pathways by regulating key proteins that affect gene expression,cell biosynthesis,DNA template transcription,etc.