中兽医医药杂志2024，Vol.43Issue(3)：15-20,6.DOI:10.13823/j.cnki.jtcvm.2024.020
茯苓多糖散质量控制研究
Quality control of Pachymaran powder
摘要
Abstract
Pachymaran is the main active component of Pachymaran powder to regulate immune function.The content of pachymaran in Pachymaran powder was determined with phenol-sulfuric acid method by spectrophotometry.The optimum test conditions were selected from six aspects:solvent,wavelength,reaction temperature,reaction time,addition amount of 5%phenol solution and addition amount of sulfuric acid.The linear relationship,precision,stability,repeatability and sample recovery method were further verified.The results showed that the optimal test conditions for the determination of Pachymaria polysaccharide were 50%sulfuric acid solution in solvent,the determination wavelength was 490 nm,the reaction time was 40 minutes at 25℃ water bath,the addition amount of 5%phenol was 1.8 mL,and the addition amount of sulfuric acid was 6.0 mL.D-anhydrous glucose had good linear relationship in the range of 10.19 μg/mL to 61.14 μg/mL(R2=0.999 4,n=6),and the average recovery was 99.31%.The method has the advantages of high sensitivity,precision,stability,good repeatability,accuracy and reliability,and can be used as a control method for polysaccharide content of Pachymaran powder.关键词
茯苓多糖散/茯苓多糖/紫外分光光度法/苯酚-硫酸法Key words
Pachymaran powder/pachymaran/ultraviolet spectrophotometry/phenol-sulfuric acid process分类
农业科技引用本文复制引用
张传津,张志民,牛华星,梁萌,李有志,陈志强..茯苓多糖散质量控制研究[J].中兽医医药杂志,2024,43(3):15-20,6.基金项目
李有志泰山产业领军人才支持计划(No.tscx202211023) （No.tscx202211023）
