中兽医医药杂志2024，Vol.43Issue(3)：33-38,封3,7.DOI:10.13823/j.cnki.jtcvm.2024.018
荆鲜止痒涂剂治疗犬皮肤瘙痒症的作用机制探讨
The mechanism of Jingxian Zhiyang Tuji in treating canis skin pruritus
赖佳欣 1秦俊杰 2田春莲 1王新宇 1沈秀帅 2王秀敏3
作者信息
- 1. 沈阳农业大学动物科学与医学学院,辽宁沈阳 110866
- 2. 北京生泰尔科技股份有限公司,北京 102600
- 3. 沈阳农业大学动物科学与医学学院,辽宁沈阳 110866||北京生泰尔科技股份有限公司,北京 102600
- 折叠
摘要
Abstract
In order to investigate the mechanism of action of Jingxian Zhiyang Tuji in the treatment of canis skin pruritus,network pharmacology method was adopted in this experiment.The active ingredients and their targets were screened by TCMSP,and all the targets were imported into Uniport database to form a drug-composition-target network.Genes related to canis skin diseases and pruritus were searched from GeneCards and OMIM databases.After intersection with the target of active drug ingredients,protein-protein interaction was mapped using STRING database and Cytoscape3.9 software.PPI network map was constructed and core targets were screened after visualization.Finally,GO and KEGG enrichment analysis was performed on common targets of drugs and diseases using DAVID database.The predicted active ingredients and core targets were verified by molecular docking technology.The results showed that 146 active ingredients were obtained,and the main components were quercetin,luteolin,apigenin,baicalein,β-sitosterol,cetoposide,sophorine,hypericin and isohesperetin.There were 55 targets associated with canine pruritus,among which the core targets were TNF,IL-6,VEGFA,etc.The core target is involved in the regulation of biological processes such as cytokine activity,extracellular space,apoptosis process,and positive regulation of RNA polymeraseⅡpromoter transcription,and acts on the regulation of MAPK signaling pathway,cytokine-cytokine receptor interaction signaling pathway,IL-17 signaling pathway and other signaling pathways in the anti-inflammatory,antibacterial,antioxidant and other processes to alleviate canis skin pruritus symptoms.The results of molecular docking showed that the key components of the drug had good binding with the core target.It showed that the treatment of canis pruritus with Jingxian Zhiyang Tuji paint involves multi-component,multi-target and multi-pathway mechanisms.关键词
荆鲜止痒涂剂/网络药理学/分子对接/犬皮肤瘙痒症/作用机制Key words
Jingxian Zhiyang Tuji/network pharmacology/molecular docking/canis skin pruritus/mechanism分类
农业科技引用本文复制引用
赖佳欣,秦俊杰,田春莲,王新宇,沈秀帅,王秀敏..荆鲜止痒涂剂治疗犬皮肤瘙痒症的作用机制探讨[J].中兽医医药杂志,2024,43(3):33-38,封3,7.
