中兽医医药杂志2024，Vol.43Issue(3)：74-78,5.DOI:10.13823/j.cnki.jtcvm.2024.037
超高效液相色谱-串联质谱法测定牛奶中4,4'-硫代二苯酚残留量
Determination of 4,4'-dihydroxydiphenyl sulfide residues in milk by ultra-high performance liquid chromatography-tandem mass spectrometry
徐坤 1赵丰华 2刘霄飞 2杨修镇 2薄永恒2
作者信息
- 1. 山东省胶州市农业农村局,山东胶州 266300
- 2. 山东省畜产品质量安全中心,山东济南 250100
- 折叠
摘要
Abstract
Ultra-high performance liquid chromatography-tandem mass spectrometry method and optimized conditions for the determination of 4,4'-dihydroxydiphenyl residues in milk were established.Milk samples were extracted with acetonitrile,defatted with sodium chloride,and purified with solid-phase extraction column.Quantification of the analyte was achieved by high performance liquid chromatography-mass spectroscopy using external standard.The results indicated that the correlations were greater than 0.99 at the range of 1.0 to 20.0 ng/mL.The limit of detection of 4,4'-dihydroxydiphenyl was 0.5 μg/kg,and the limit of quantification was 1.0 μg/kg.The average recoveries of 4,4'-dihydroxydiphenyl ranged from 60%to 90%at spiled levels of 1.0 to 5.0 μg/kg with RSD less than 10%.The experiment show that the method presents low detection limit,high recovery,qualitative standard for detection of 4,4'-dihydroxydiphenyl residues in milk.关键词
牛奶/4,4'-硫代二苯酚/残留量/超高效液相色谱-串联质谱法Key words
milk/4,4'-dihydroxydiphenyl/residue/ultra-high performance liquid chromatography-tandem mass spectrometry分类
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徐坤,赵丰华,刘霄飞,杨修镇,薄永恒..超高效液相色谱-串联质谱法测定牛奶中4,4'-硫代二苯酚残留量[J].中兽医医药杂志,2024,43(3):74-78,5.
