中药材2024，Vol.47Issue(1)：81-84,4.DOI:10.13863/j.issn1001-4454.2024.01.014
苗药了哥王中瑞香烷二萜原酸酯类成分及其抗肿瘤活性研究
Daphnane Diterpenoid Orthoesters of Miao Medicine Wikstroemia indica and the Antitumor Activities
摘要
Abstract
Objective:To study the daphnane diterpenoid orthoesters of Miao Medicine Wikstroemia indica and the antitumor activi-ties.Methods:The daphnane diterpenoid orthoesters of Wikstroemia indica were isolated and purified by silica gel column chromatogra-phy,RP-C18 reversed phase column chromatography,Sephadex LH-20 glucan gel column chromatography and semi-preparative liquid chromatography,etc.The structures were identified by the physicochemical characteristics and spectral data.The cytotoxic activities of compounds 1,3,5 against hepatoma HepG2 cells line were evaluated by MTT method.Results:Five daphnane diterpenoid orthoesters were isolated from Miao medicine Wikstroemia indica and identified as 6α,7α-dihydyoxyhuratoxin(1),simplexin(2),12β-acetoxyhura-toxin(3),excoecafolin D(4),pimelea factor P2(5).These compounds showed the significant cytotoxicities against HepG2 cells.Conclu-sion:Among them,compound 1 is a new compound,compounds 2 to 5 are isolated from Wikstroemia indica for the first time.Compound 3 has significant effect on inhibition of HepG2 cell proliferation with IC50 value of(5.75±0.15)μg/mL.关键词
苗药/了哥王/瑞香烷二萜原酸酯/抗肿瘤活性Key words
Miao medicine/Wikstroemia indica(L.)C.A.Mey./Daphnane diterpenoid orthoesters/Antitumor activity分类
医药卫生引用本文复制引用
国光梅,谭琳,苑春茂,穆淑珍,汪冶..苗药了哥王中瑞香烷二萜原酸酯类成分及其抗肿瘤活性研究[J].中药材,2024,47(1):81-84,4.基金项目
贵州省科技支撑计划项目(黔科合支撑[2021]一般110) （黔科合支撑[2021]一般110）
