中药材2024，Vol.47Issue(1)：117-123,7.DOI:10.13863/j.issn1001-4454.2024.01.021
山银柴胡质量标准研究
Study on Quality Standard of Gypsophilae Radix
摘要
Abstract
Objective:To study the quality evaluation standard of Gypsophilae Radix,to establish the fingerprint analysis method.Methods:Water content,total ash content,acid insoluble ash content and ethanol soluble extract content of Gypsophilae Radix were de-termined according to the relevant methods in the appendix of Chinese Pharmacopoeia(2020 edition).HPLC method was used to deter-mine the content of index components in Gypsophilae Radix.The fingerprint of 30 batches of Gypsophilae Radix was established,and the quality was evaluated by similarity evaluation,cluster analysis,orthogonal partial least squares discriminant analysis(OPLS-DA).Re-sults:The mean values of water,total ash,acid insoluble ash,ethanol soluble extract,methyl gypsogenin 3-O-β-D-glucuronopyranoside,gypsogenin and quillaic acid were 8.89％,12.67％,0.79％,34.53％,13.58 mg/g,1.62 mg/g,1.03 mg/g,respectively.A total of 17 common peaks were found in 30 batches of Gypsophilae Radix fingerprint.The similarity of 30 batches of samples ranged from 0.916 to 0.997.The samples were divided into three categories by cluster analysis,and seven different components were screened out by OPLS-DA analysis.Conclusion:The method established in this study can provide reference for the establishment of quality standard of Gyp-sophilae Radix.关键词
山银柴胡/长蕊石头花/细叶石头花/质量标准/指纹图谱Key words
Gypsophilae Radix/Gypsophila oldhamiana Miq./Gypsophila licentiana Hand.-Mazz./Quality standard/Fingerprint分类
医药卫生引用本文复制引用
张旭,张飞,郭凯华,田志浩,郜志宁,陈随清..山银柴胡质量标准研究[J].中药材,2024,47(1):117-123,7.基金项目
2019年中医药公共卫生服务补助专项"全国中药资源普查项目"(豫财社[2019]40号) （豫财社[2019]40号）
河南省中药材产业技术体系(豫农科教[2018]14号) （豫农科教[2018]14号）
