臭氧急性暴露对大鼠肺组织损伤的影响OACSTPCD

OBJECTIVE:To develop a rat model for investigating effects of acute ozone exposure on lung tissue injury in rats.METHODS:48 SPF-grade SD rats,half male and half female,were randomly divided into 4 groups:control,and 1.0,2.0 and 4.0 ppm exposures.The environmental exposure was for 4 h every day for 7 d.After exposure,lung tissues and blood were collected,and changes of lung coefficient were calculated.Oxidative stress indexes of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in lung tissue homogenate were measured.Changes of serum inflammatory factors(IL-1β and TNF-α)and pathological status of lung tissues were detected.RESULTS:Compared with the control group,the lung coefficient of rats increased gradually under different concentrations of ozone exposure(P<0.05).In addition,MDA content in lung tissues increased with increased concentrations(P<0.01).SOD content in lung tissue of female rats was increased(P<0.01),and SOD content in lung tissue of male rats was first increased and then decreased(P<0.05).The content of GSH-Px was increased(P<0.01).The serum levels of TNF-α in female rats were increased(P<0.05),and the levels of IL-1β and TNF-α in male rats were increased(P<0.05).Histopathological results showed that the alveolar septum of lung tissues in female rats was thickened and there was inflammatory cell infiltration.Thickening of the alveolar septum of the 2.0 and 4.0 ppm groups was worse than that of the control group.In the control group,the pulmonary alveolar septum was slightly thickened and there was mild inflammation.The thickening of alveolar interval in each ozone dose group was more severe than that in the control group,and large-scale lung tissue consolidation was observed in the 2.0 and 4.0 ppm dose groups.CONCLUSION:Ozone exposure caused pathological injury,oxidative stress and inflammation in rat lung tissues,and the toxic effects showed sex gender differences.