癌变·畸变·突变2024，Vol.36Issue(3)：219-223,5.DOI:10.3969/j.issn.1004-616x.2024.03.008
臭氧急性暴露对大鼠肺组织损伤的影响
Effects of acute exposure to ozone on lung tissue injury in rats
摘要
Abstract
OBJECTIVE:To develop a rat model for investigating effects of acute ozone exposure on lung tissue injury in rats.METHODS:48 SPF-grade SD rats,half male and half female,were randomly divided into 4 groups:control,and 1.0,2.0 and 4.0 ppm exposures.The environmental exposure was for 4 h every day for 7 d.After exposure,lung tissues and blood were collected,and changes of lung coefficient were calculated.Oxidative stress indexes of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in lung tissue homogenate were measured.Changes of serum inflammatory factors(IL-1β and TNF-α)and pathological status of lung tissues were detected.RESULTS:Compared with the control group,the lung coefficient of rats increased gradually under different concentrations of ozone exposure(P<0.05).In addition,MDA content in lung tissues increased with increased concentrations(P<0.01).SOD content in lung tissue of female rats was increased(P<0.01),and SOD content in lung tissue of male rats was first increased and then decreased(P<0.05).The content of GSH-Px was increased(P<0.01).The serum levels of TNF-α in female rats were increased(P<0.05),and the levels of IL-1β and TNF-α in male rats were increased(P<0.05).Histopathological results showed that the alveolar septum of lung tissues in female rats was thickened and there was inflammatory cell infiltration.Thickening of the alveolar septum of the 2.0 and 4.0 ppm groups was worse than that of the control group.In the control group,the pulmonary alveolar septum was slightly thickened and there was mild inflammation.The thickening of alveolar interval in each ozone dose group was more severe than that in the control group,and large-scale lung tissue consolidation was observed in the 2.0 and 4.0 ppm dose groups.CONCLUSION:Ozone exposure caused pathological injury,oxidative stress and inflammation in rat lung tissues,and the toxic effects showed sex gender differences.关键词
臭氧/肺损伤/炎症反应/急性暴露/性别差异Key words
ozone/lung injury/inflammatory response/acute exposure/gender difference分类
医药卫生引用本文复制引用
孙小宁,苏德奇,杨浩峰,田亚绒,刘慧,黄小溪..臭氧急性暴露对大鼠肺组织损伤的影响[J].癌变·畸变·突变,2024,36(3):219-223,5.基金项目
新疆维吾尔自治区自然科学基金(2022D01C702) （2022D01C702）
新疆医科大学医学科学研究所开放课题(YXYJ2023101) （YXYJ2023101）
新疆特殊环境与健康重点实验室项目(SKL-SEHR-2021-06) （SKL-SEHR-2021-06）
