中医药临床杂志2024，Vol.36Issue(5)：918-924,7.DOI:10.16448/j.cjtcm.2024.0522
黄芪治疗糖尿病合并冠心病的作用机制探讨
Study on the Mechanism of Astragalus in Treating diabetes Complicated with Coronary Heart Disease Based on Network Pharmacology and Molecular Docking
摘要
Abstract
Objective:Use network pharmacology analysis method to explore the possible targets and mechanisms of Astragalus in treating diabetes complicated with coronary heart disease.Methods:Use the TCMSP platform and Uni-prot database to search and screen the active ingredients of Astragalus membranaceus and their corresponding targets;search for the disease targets of diabetes combined with coronary heart disease in the GeneCards database and OMIM database,and obtain the Astragalus membranaceus-active ingredients-target genes through Cytoscape 3.9.1 Visual network diagram;submit the disease and drug corresponding targets to Venny2.1.0 to generate intersecting targets,and build a protein interaction(PPI)network through the protein interaction(STRING)platform.Based on the Metascape 3.9.1 database,GO enrichment analysis and KEGG pathway enrichment analysis were performed to identify the biolog-ical processes and signaling pathways that may be involved.Finally,molecular docking technology was used to briefly verify the above conjecture.Results:A total of 17 key compounds of Astragalus,195 corresponding targets,and 3815 disease-related targets were obtained,which were imported into Venny2.1.0 to generate 163 intersection targets.The enrichment analysis results show that astragalus'treatment of diabetes complicated with coronary heart disease mainly involves hormone response(response to hormone),cellular response to organic cyclic compound(cellular response to organic cyclic compound),cellular response to lipid(cellular response to lipid),and inorganic matter response.(response to inorganic substance)and other biological processes;may involve pathways in cancer,lipid and atheroscierosis,and AGE-RAGE signaling pathway in diabetic complications.diabetic complications),P13K-AKt signaling pathway(P13K-AKt signaling pathway)and other signaling pathways.Molecular docking results indicate that the active drug molecule has a good combination with the core target.Conclusion:This study reveals that astragalus may be involved in inhibiting insulin resistance,regulating glucose and lipid metabolism and many other biological processes in the body,and achieves its therapeutic effect on diabetes complicated with coronary heart disease through multiple components,multiple targets and multiple pathways.关键词
黄芪/糖尿病合并冠心病/网络药理学Key words
Astragalus membranaceus/Diabetes complicated with coronary heart disease/Network pharmacology分类
医药卫生引用本文复制引用
张瑞林,郑曙琴..黄芪治疗糖尿病合并冠心病的作用机制探讨[J].中医药临床杂志,2024,36(5):918-924,7.基金项目
全国名老中医专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
