成都中医药大学学报2024，Vol.47Issue(3)：8-11,4.DOI:10.13593/j.cnki.51-1501/r.2024.03.08
冬凌草甲素对海马CA1星形胶质细胞P2X7受体电流的影响
Effect of Oridonin on P2X7 Receptor Agonist-induced Current Response in Hippocampal CA1 Astrocytes
摘要
Abstract
Objective:To explore whether oridonin has an effect on P2X7 receptor agonist-induced current in hippocampal CA1 astro-cytes.Methods:Autodock software wasused to perform molecular docking between oridonin and P2X7 protein;whole cell patch clamp to record the effect of oridonin on P2X7 receptor agonist Bz-ATP induced current responseof hippocampal CA1 astrocytes.Results:The binding energy between oridonin and P2X7 protein is-5.86 kcal/mol,which can form hydrogen bonds with the Lys592 residue of P2X7 protein and hydrophobic interactions with Tyr550 residue.Bz-ATP could stimulate a current response of astrocytes in the CA1 region of hippocampus,and the current response stimulated by 1000 pmol/L Bz-ATP is much stronger.1 hour of incubation with 10 p,mol/L oridonin solution decreased Bz-ATP induced current response of astrocyte significantly(P＜0.05),while glutamate receptor agonists NMDA and AMPA have no influence on that.Conclusion:oridonin can dock with P2X7 protein to form a stable complex with good af-finity,and inhibit P2X7 receptor agonist Bz-ATP induced current response in hippocampal CA1 astrocytes.关键词
冬凌草甲素/P2X7受体/分子对接/膜片钳/海马星形胶质细胞Key words
Oridonin/P2X7 receptor/Molecular docking/Patch clamp/Hippocampal astrocytes分类
医药卫生引用本文复制引用
赵亚飞,吴嘉思,童宇,睢婉婉,时政..冬凌草甲素对海马CA1星形胶质细胞P2X7受体电流的影响[J].成都中医药大学学报,2024,47(3):8-11,4.基金项目
中国博士后基金(BX20220049,2022MD713682) （BX20220049,2022MD713682）
成都大学附属医院院级课题项目(Y2021006,Y202232,Y202234) （Y2021006,Y202232,Y202234）
成都大学临床医学院/附属医院创新团队项目(CDFYCX202208) （CDFYCX202208）
