成都中医药大学学报2024，Vol.47Issue(3)：12-17,6.DOI:10.13593/j.cnki.51-1501/r.2024.03.12
电针调控CD73及腺苷信号对脓毒症急性肾损伤的影响
Effect of Eletroacupuncture on CD73 and Adenosine Signaling in Sepsis-Associated Acute Kidney Injury
摘要
Abstract
Objective:To observe the effect of electroacupuncture on sepsis-associated acute kidney injury(SA-AKI)and clarify the link between electroacupuncture(EA)to improve SA-AKI and the regulation of CD73 and adenosine signaling.Methods:C57BL/6J mice were randomly divided into Control,LPS,EA and shamEA group.The murine model of acute kidney injury was established by an intraperitoneal injection of LPS.EA stimulation was performed 12 hours before and after intraperitoneal injection of LPS and applied at the bilateral Zusanli(足三里,ST36)acupoint,with a parameter of 10 Hz and 3.0 mA for an amount of time equaling 30 minutes each.The content of the Tumor Necrosis Factor Alpha(TNF-α)in kidney tissue was quantified by the Enzyme-Linked Immunosorbent Assay(ELISA)kits.Meanwhile,the protein expression of IL-6,CD73,A2AR,and HIF-1α in kidney tissue was extracted from the Western Blot(WB).Results:Compared with Control group,the content of TNF-α and the protein expression of IL-6 in kidney tissue were significantly increased in LPS group(P＜0.001).In contrast,the EA group has a significant reduction in the level of TNF-α and protein expression of IL-6 compared with the LPS group(P＜0.001).There was a significantly low protein expression of CD73 and A2AR in LPS group(P＜0.001),in comparison to the Control group,whereas that of HIF-1α was significantly higher.While in EA group,the expression of CD73 and A2AR increased dramatically(P＜0.001),but HIF-1α protein expression decreased significantly compared to the LPS group(P＜0.001).Conclusion:Electroacupuncture,hence,through downregulation of IL-6 protein expression and TNF-α content in kidney tissues of the mouse model with SA-AKI,exerts anti-inflammatory effects.The efficacy of electroacupunc-ture to ameliorate SA-AKI may be associated with modulation of CD73 and downstream adenosine signaling.关键词
电针/抗炎效应/脓毒症急性肾损伤/腺苷/胞外-5'-核苷酸酶/腺苷信号A2A受体Key words
Electroacupuncture/Anti-inflammatory effect/Sepsis-associated acute kidney injury/Adenosine/CD73/A2AR分类
医药卫生引用本文复制引用
程辞云,杨玲玲,胡秀敏,陈莎莎,王蕊,余常..电针调控CD73及腺苷信号对脓毒症急性肾损伤的影响[J].成都中医药大学学报,2024,47(3):12-17,6.基金项目
四川省中医药管理局中医药科研专项课题(2021MS103 （）
2021MS231) （）
四川省自然科学基金项目(2022NSFSC0852) （2022NSFSC0852）
