动物医学进展2024，Vol.45Issue(7)：18-23,6.
新疆某规模化肉牛场牛病毒性腹泻病毒分离与基因型鉴定
Isolation and Genotype Identification of Bovine Viral Diarrhea Virus from A Large-scale Beef Cattle Farm in Xinjiang
摘要
Abstract
Many large-scale cattle farms in Xinjiang are infected with bovine viral diarrhea virus(BVDV),and the genotypes are diverse,which brings some difficulties to the prevention and control of the disease,vaccine selection and purification.The genotyping of large-scale cattle farms in Xinjiang plays an important guiding role in the prevention and control of BVDV.In this test,calf anticoagulant samples,confirmed posi-tive for BVDV colloidal gold antigen detection card,were collected and lymphocytes were isolated.Virus i-solation was performed on MDBK monolayer cells,blind transmission for five generations,and trophic RNA was extracted for reverse transcript polymerase chain reaction(RT-PCR)analysis with BVDV 5'-UTR and Npro-specific primers.The results showed that one BL isolate,belonging to the BVDV-lm sub-type,and this study enriched the molecular epidemiology database of BVDV in large-scale cattle farms in southern Xinjiang.关键词
牛病毒性腹泻病毒/基因型鉴定/逆转录-聚合酶链反应Key words
Bovine viral diarrhea virus/genotype identification/RT-PCR分类
农业科技引用本文复制引用
黄琴,陈红强,张怡淼,马颖超,胡建军,张伟..新疆某规模化肉牛场牛病毒性腹泻病毒分离与基因型鉴定[J].动物医学进展,2024,45(7):18-23,6.基金项目
兵团塔里木动物疫病诊断与防控工程实验室项目(ELDC202304) （ELDC202304）
兵团科技局现代农业科技攻关与成果转化计划项目(2016AC012) （2016AC012）
