动物医学进展2024，Vol.45Issue(7)：24-30,7.
鸭肠炎病毒和A型产气荚膜梭菌共感染绿壳蛋鸭十二指肠代谢组学分析
Duodenal Metabolomic Analysis of DEV and CpA Co-infected Green-shelled Ducks
摘要
Abstract
The aim of this experiment was to investigate the changes of differential metabolites in the duode-num at 114 h of co-infection of green-shelled egg-laying ducks with duck enteritis virus(DEV)and Clos-tridium perfringens type A(CpA).Twelve healthy green-shelled egg ducks were selected for the experi-ment and randomly divided into the control group(CK)and the co-infected group(TCD).We determined the success of DEV and CpA co-infection through clinical observation,pathologic observation by dissection and PCR,and then examined the metabolite changes in the duodenum of ducks in the CK group and TCD groups at 114 h of infection by liquid chromatography-mass spectrometry(LC-MS),in order to screen for the potential differential metabolites and metabolic pathways.The results showed that 29 different metabo-lites were found in the duodenum of ducks in the TCD group at 114 h of infection compared with those in the CK group,including 5'-s-methyl-5'-thioadenosine,cytidine,deoxycorticosterone 21-glucoside,putrescine and 2-oxoindole,putrescine,naringenin,2-oxoindole,and indole-3-glycolide,etc.The metabolic pathways,mainly nicotinic acid,niacin,niacin,2-oxoindole,and indole-3-glycolide,were significantly enriched into 22 metabolic pathways via KEGG enrichment.Metabolic pathways,mainly nicotinic acid ester and nicotinam-ide metabolism,cysteine and methionine metabolism,tyrosine metabolism,tryptophan metabolism,glycer-ophospholipid metabolism,etc.Among them,those related to the body's immunity and inflammation were putrescine,naringenin,2-oxoindole,indole-3-glycolaldehyde,5'-s-methyl-5'-thioadenosine,nicotinic acid es-ter,cysteine Gmethionine metabolism,tryptophan metabolism and nicotinamide metabolism.In summary,DEV and CpA co-infection in green-shelled ducks may affect duodenal inflammation in green-shelled ducks by influencing metabolites such as putrescine and naringenin,as well as tryptophan metabolism and cyste-ine-methionine metabolism pathways,which provides a scientific basis for elucidating the mechanism of co-infection with DEV and CpA.关键词
鸭肠炎病毒/A型产气荚膜梭菌/代谢组学/十二指肠Key words
DEV/CpA/metabolomics/duodenum分类
农业科技引用本文复制引用
张晨旭,田琴,毕文文,王开功,周碧君,文明..鸭肠炎病毒和A型产气荚膜梭菌共感染绿壳蛋鸭十二指肠代谢组学分析[J].动物医学进展,2024,45(7):24-30,7.基金项目
国家自然科学基金项目(315060703) （315060703）
贵州省百层次创新型人才项目(黔科合平台人才[2016]6009号) （黔科合平台人才[2016]6009号）
贵州省科技平台项目(黔科合平台人才[2018]5253号) （黔科合平台人才[2018]5253号）
