动物医学进展2024，Vol.45Issue(7)：42-47,6.
新疆阿克苏和吐鲁番地区硬蜱中3种立克次体检测及遗传进化分析
Detection and Genetic Evolution Analysis of Three Species of Rickettsia in Ticks of Aksu and Turpan Areas
摘要
Abstract
This article analyzes 132 tick samples collected from Aksu(SY)and Turpan(ADH)to study the genetic evolution relationship between ticks and their carrying Candidatu Midichloria mitochondrii and pathogens.A total of 132 total DNA samples were extracted from a single tick,and PCR was used to ampli-fy the 16SRNA gene of tick A.bovis in 132 DNA samples.Subsequently,7 positive samples were selected for molecular biology identification based on COI gene;Finally,MEGA X was used to construct a phyloge-netic tree.DNA SP V5.0 software was used to count the number of haplotypes of ticks in two locations,cal-culate mutation sites and nucleotide diversity.The total infection rate of Anaplasma in ticks was 34.8％(46/132)through PCR detection;The sequencing results showed that SY1 was a R.turanicus carrying potted fever Rickettsia,SY2,SY3,and SY4 were H.asiaticums,with SY2 and SY3 carrying Ca.M.mito-chondrii sp.and SY4 carrying A.phagocytophilum;ADH1,ADH2,and ADH3 are H.anatolicums.ADH1 carries A.ovis,while ADH2 and ADH3 carry Ca.M.mitochondrii sp.By constructing phylogenetic tree a-nalysis,it was found that Candidatus Midichloria mitochondrii sp.(Ca.M.mitochondrii sp.)identified in ADH2,ADH3,SY2,and SY3 had high homology with the Italian strain Ca.M.mitochondrii,clustered into one branch.Ana plasma sp.identified in SY4 clustered into one branch with the Xinjiang strain Anaplasma phagocytophilum,and Anaplasma sp.identified in ADH1 clustered into one branch with the Italian strain Anaplasma ovis;SY1 shows a close genetic relationship with spotted fever Rickettsia;Analysis of haplo-type results showed that the number of haplotypes in H.asiaticum is 2,number of variable sites is 4,and the nucleic acid diversity is 0.004 08.The number of haplotypes in H.anatolicum is 3,number of variable sites is 3,and the nucleic acid diversity is 0.003 03.The gene flow of H.asiaticum and H.anatolicum in the two regions is relatively low.In this study,Ca.M.mitochondrii was detected in both H.asiaticum and H.anatolicum species can provide a theoretical basis for the relationship between tick borne diseases and Ca.M.mitochondrii in the future.关键词
蜱传疾病/共生菌/进化分析Key words
tick borne disease/Candidatus Midichloria mitochondrii/evolutionary analysis分类
农业科技引用本文复制引用
温丽翠,李才善,周娜,赵珊珊,石文玉,普浩,刘诗语,伍军,巴音查汗..新疆阿克苏和吐鲁番地区硬蜱中3种立克次体检测及遗传进化分析[J].动物医学进展,2024,45(7):42-47,6.基金项目
国际合作项目新疆维吾尔自治区区域协同创新专项-上海合作组织科技伙伴计划及国际科技合作计划项目(2021E01001) （2021E01001）
新疆维吾尔自治区的研究生创新项目(XJ2023G142) （XJ2023G142）
