动物医学进展2024,Vol.45Issue(7):139-144,6.
宁夏地区绵羊肺炎支原体感染的流行情况调查
Prevalence Investigation on Mycoplasma ovipneumoniae Infection in Ningxia Region
摘要
Abstract
In order to preliminarily grasp the prevalence of Mycoplasma ovipneumoniae(Mo)infection in sheep(Tan sheep)in Ningxia Hui Autonomous Region,this study used the Mo TaqMan-MGB probe real-time fluorescence quantitative PCR detection method established in our laboratory to collect 400 sheep na-sal swab samples from different counties/districts in Ningxia from 2022 to 2023 for real-time fluorescence quantitative PCR detection results of Mo infection in Tan sheep in Ningxia.The results showed that the molecular biological detection results of Mo in Tongxin county,Lingwu county,Pingluo county,Yongning county,Helan county,Litong district and Yanchi county of 7 counties/districts in Ningxia were positive.In this experiment,a total of 400 nasal swabs of Tan sheep were randomly collected,and 329 were positive for Mo,with an average positive detection rate of 82.25%.The positive rates of Mo in Tongxin county,Lingwu county,Pingluo county,Yongning county,Helan county,Litong district and Yanchi county were 80.32%,93.42%,79.59%,78.18%,82.25%,80.43%and 76.47%,respectively.The positive rate of Mo in Lingwu county was the highest,which was significantly different from that in other counties(P<0.01),and Yan-chi county was the lowest.The highest detection rate of Mo was 100.00%in 3-5 months old,which was sig-nificantly different from that in 1-2 months old and 2-3 years old(P<0.01).The detection rate of Mo in 6-8 months old sheep was the second,which was significantly different from that in 1-2 months old and 2-3 years old sheep(P<0.01).The lowest infection rate of Mo was 10.00%within 1 month old,which was significantly different from that of 3-5 months old and 2-3 years old sheep(P<0.05).关键词
滩羊/绵羊肺炎支原体/调查Key words
Tan sheep/Mycoplasma ovipneumoniae/investigation分类
农业科技引用本文复制引用
刘红燕,王建东,何生虎,郭亚男..宁夏地区绵羊肺炎支原体感染的流行情况调查[J].动物医学进展,2024,45(7):139-144,6.基金项目
宁夏回族自治区重点研发项目(2021BEF02026) (2021BEF02026)
同心县2023年科技项目(2023KJJH002) (2023KJJH002)