南方医科大学学报2024，Vol.44Issue(5)：801-809,9.DOI:10.12122/j.issn.1673-4254.2024.05.01
正常小鼠血清通过抑制focal adhesion信号通路减轻小鼠放射性肺炎
Normal mouse serum alleviates radiation pneumonitis in mice by inhibiting the focal adhesion signaling pathway
摘要
Abstract
Objective To evaluate the therapeutic effect of normal mouse serum on radiation pneumonitis in mice and explore the possible mechanism.Methods Mouse models of radiation pneumonitis induced by thoracic radiation exposure were given intravenous injections of 100 μL normal mouse serum or normal saline immediately after the exposure followed by injections once every other day for a total of 8 injections.On the 15th day after irradiation,histopathological changes of the lungs of the mice were examined using HE staining,the levels of TNF-α,TGF-β,IL-1α and IL-6 in the lung tissue and serum were detected using ELISA,and the percentages of lymphocytes in the lung tissue were analyzed with flow cytometry.High-throughput sequencing of exosome miRNA was carried out to explore the changes in the signaling pathways.The mRNA expression levels of the immune-related genes were detected by qRT-PCR,and the protein expressions of talin-1,tensin2,FAK,vinculin,α-actinin and paxillin in the focal adhesion signaling pathway were detected with Western blotting.Results In the mouse models of radiation pneumonitis,injections of normal mouse serum significantly decreased the lung organ coefficient,lowered the levels of TNF-α,TGF-β,IL-1α and IL-6 in the serum and lung tissues,and ameliorated infiltration of CD45+,CD4+and Treg lymphocytes in the lung tissue(all P<0.05).The expression levels of Egfr and Pik3cd genes at both the mRNA and protein levels and the protein expressions of talin-1,tensin2,FAK,vinculin,α-actinin and paxillin were all significantly down-regulated in the mouse models after normal mouse serum treatment.Conclusion Normal mouse serum ameliorates radiation pneumonitis in mice by inhibiting the expressions of key proteins in the Focal adhesion signaling pathway.关键词
正常小鼠血清/放射性肺炎/外泌体/粘着斑途径Key words
normal mouse serum/radiation pneumonitis/exosomes/focal adhesion pathway引用本文复制引用
苑通,郭玉莹,张俊伶,樊赛军..正常小鼠血清通过抑制focal adhesion信号通路减轻小鼠放射性肺炎[J].南方医科大学学报,2024,44(5):801-809,9.基金项目
Supported by the CAMS Innovation Fund for Medical Sciences(CIFMS,2021-I2M-1-042,2022-I2M-2-003)and National Natural Science Foundation of China(82273577). 中国医学科学院医学与健康科技创新工程重大协同创新项目(2021-I2M-1-042) （CIFMS,2021-I2M-1-042,2022-I2M-2-003）
中国医学科学院国家医学健康科技战略平台与体系建设项目(2022-I2M-2-003) （2022-I2M-2-003）
国家自然科学基金(82273577) （82273577）
