南方医科大学学报2024，Vol.44Issue(5)：841-850,10.DOI:10.12122/j.issn.1673-4254.2024.05.05
LncRNA FEZF1-AS1通过miR-130a-5p/CCND1轴促进非小细胞肺癌发展的分子机制研究
Overexpression of lncRNA FEZF1-AS1 promotes progression of non-small cell lung cancer via the miR-130a-5p/CCND1 axis
摘要
Abstract
Objective To explore the molecular mechanism by which FEZF1-AS1 overexpression promotes progression of non-small cell lung cancer(NSCLC)via the miR-130a-5p/CCND1 axis.Methods TCGA database was used to analyze FEZF1-AS1 expression levels in NSCLC.FEZF1-AS1 expression was detected by qRT-PCR in clinical specimens of NSCLC tissues and NSCLC cell lines,and its correlation with clinical features of the patients were analyzed.The binding sites of FEZF1-AS1 with hsa-miR-130a-5p and those of hsa-miR-130a-5p with CCND1 were predicted.CCK8 assay,clone formation assay,scratch assay,and Transwell assay were employed to examine the effects of FEZF1-AS1 knockdown and hsa-miR-130a-5p inhibitor on proliferation,invasion,and migration abilities of lung cancer cell lines.Dual luciferase assay was used to verify the binding of FEZF1-AS1 with hsa-miR-130a-5p and the binding of hsa-miR-130a-5p with CCND1.Western blotting was performed to detect the changes in CCND1 protein expression level in H1299 and H358 cells following FEZF1-AS1 knockdown and treatment with hsa-miR-130a-5p inhibitor.Results FEZF1-AS1 was highly expressed in NSCLC tissues in close correlation with lymph node metastasis and also in H1299 and H358 cell lines(all P<0.05).FEZF1-AS1 knockdown obviously reduced proliferation,migration,and invasion abilities of NSCLC cells(P<0.05).Dual luciferase assay confirmed the binding of hsa-miR-130a-5p with FEZF1-AS1 and CCND1(P<0.05),and hsa-miR-130a-5p inhibitor significantly inhibited proliferation,migration,and invasion of NSCLC cells(P<0.05).FEZF1-AS1 knockdown significantly reduced CCND1 protein expression in NSCLC cells,and this effect was strongly inhibited by treatment with hsa-miR-130a-5p inhibitor(P<0.05).Conclusion FEZF1-AS1 is highly expressed in NSCLC tissue in close correlation with lymph node metastasis to promote cancer progression through the miR-130a-5p/CCND1 axis.关键词
非小细胞肺癌/长链非编码RNA/FEZF1-AS1/miR-130a-5p/CCND1Key words
non-small cell lung cancer/lncRNA/FEZF1-AS1/miR-130a-5p/CCND1引用本文复制引用
李菲凡,向俊馨,刘佳慧,王效静,江浩..LncRNA FEZF1-AS1通过miR-130a-5p/CCND1轴促进非小细胞肺癌发展的分子机制研究[J].南方医科大学学报,2024,44(5):841-850,10.基金项目
国家自然科学基金(82373329) （82373329）
安徽省教育厅自然科学研究重点项目(KJ2018A0997) Supported by National Natural Science Foundation of China(82373329). （KJ2018A0997）
