南方医科大学学报2024，Vol.44Issue(5)：904-912,9.DOI:10.12122/j.issn.1673-4254.2024.05.12
扁蒴藤素通过活性氧调控PI3K/AKT通路增强顺铂诱导鼻咽癌细胞凋亡
Pristimerin enhances cisplatin-induced apoptosis in nasopharyngeal carcinoma cells via ROS-mediated deactivation of the PI3K/AKT signaling pathway
摘要
Abstract
Objective To explore the effect of pristimerin combined with cisplatin on proliferation and apoptosis of nasopharyngeal carcinoma cells.Methods CCK-8 assay was used to examine the survival rate of HNE-1 and CNE-2Z cells following treatment for 24 h with different concentrations of pristimerin,cisplatin or their combination.The changes in colony formation ability,apoptosis,and intracellular reactive oxygen species(ROS)levels of the treated cells were analyzed using colony formation assay and flow cytometry.Western blotting was performed to detect the changes in protein expressions in the cells.The effects of pre-treatment with NAC on proliferation,apoptosis,and PI3K/AKT signaling pathway were observed in pristimerin-and/or cisplatin-treated cells.Results Both pristimerin and cisplatin significantly lowered the survival rate of HNE-1 and CNE-2Z cells(P<0.05).Compared with pristimerin or cisplatin alone,their combination more strongly inhibited survival and colony formation ability of the cells,increased cell apoptosis rate and intracellular ROS levels,upregulated the protein expressions of Bax,cleaved caspase-3,and cleaved PARP,and downregulated the protein expressions of Bcl-2,Mcl-1,PARP and p-PI3K and p-AKT(P<0.05).NAC pretreatment significantly attenuated proliferation inhibition and apoptosis-promoting effects of pristimerin combined with cisplatin,and partially restored the downregulated protein expressions of p-PI3K and p-AKT in HNE-1 and CNE-2Z cells with the combined treatment(P<0.05).Conclusion Pristimerin can enhance cisplatin-induced proliferation inhibition and apoptosis in nasopharyngeal carcinoma cells,the mechanism of which may involve ROS-mediated deactivation of the PI3K/AKT signaling pathway.关键词
鼻咽癌/扁朔藤素/顺铂/活性氧/PI3K/AKT信号通路Key words
nasopharyngeal carcinoma/pristimerin/cisplatin/reactive oxygen species/PI3K/AKT signaling pathway引用本文复制引用
王媛媛,陈腾,从小凡,李依然,陈蕊,张配,孙小锦,赵素容..扁蒴藤素通过活性氧调控PI3K/AKT通路增强顺铂诱导鼻咽癌细胞凋亡[J].南方医科大学学报,2024,44(5):904-912,9.基金项目
国家自然科学基金(81603155) （81603155）
安徽省高校自然科学研究重点项目(KJ2021A0736,2022AH051534) （KJ2021A0736,2022AH051534）
蚌埠医学院自然科学重点项目(2021byzd018) （2021byzd018）
安徽省大学生创新创业训练项目(S202210367013,S202210367109) （S202210367013,S202210367109）
蚌埠医学院研究生科研创新计划自然科学项目(Byycx22061) Supported by National Natural Science Foundation of China(81603155). （Byycx22061）
