南方医科大学学报2024，Vol.44Issue(5)：941-949,9.DOI:10.12122/j.issn.1673-4254.2024.05.16
基于转录组学测序及生物信息学方法鉴定肠上皮化生的潜在致病基因
Identification of potential pathogenic genes of intestinal metaplasia based on transcriptomic sequencing and bioinformatics analysis
摘要
Abstract
Objective To explore the potential pathogenic genes of intestinal metaplasia.Methods Twenty-one patients with intestinal metaplasia admitted to the Department of Gastroenterology at the Second Affiliated Hospital of Anhui University of Chinese Medicine from January,2022 to June,2022,and 21 healthy subjects undergoing gastroscopic examination during the same period were enrolled in this study.All the participants underwent gastroscopy and pathological examination,and gastric tissue samples were collected for transcriptome sequencing to screen for differentially expressed genes(DEGs).The biological functions of the DEGs were analyzed using bioinformatics analysis,and qRT-PCR was used to validate the results.Results Transcriptomic sequencing identified a total of 1373 DEGs,including 827 upregulated and 546 downregulated ones.The top 6 upregulated genes(AGMAT,CCL25,FABP1,CDX1,SPINK4,and MUC2),ranked based on their significance and average expression level,were selected for validation,and qRT-PCR showed significant upregulation of their mRNAs in the gastric tissues of patients with intestinal metaplasia(P<0.05).Conclusion AGMAT,CCL25,FABP1,CDX1,SPINK4,and MUC2 participate in the occurrence and development of intestinal metaplasia,and may serve as potential biomarkers for diagnosing intestinal metaplasia.关键词
肠上皮化生/致病基因/转录组学/生物信息学Key words
intestinal metaplasia/pathogenic genes/transcriptomics sequencing/bioinformatics引用本文复制引用
裴蓓,张艺,魏思源,梅语,宋标,董港,温子昂,李学军..基于转录组学测序及生物信息学方法鉴定肠上皮化生的潜在致病基因[J].南方医科大学学报,2024,44(5):941-949,9.基金项目
安徽省重大疑难疾病中西医协同攻关项目(0708-2021) （0708-2021）
