福建农业学报2024，Vol.39Issue(3)：251-258,8.DOI:10.19303/j.issn.1008-0384.2024.03.002
甜玉米自交系重金属Pb、Cd积累差异及分子检测
Accumulation and Molecular Detection of Pb and Cd in Sweet Corn Inbred Lines
摘要
Abstract
[Objective]Sweet corn inbred lines low in accumulating Pb and Cd were identified,and a molecular detection method for the pollutants examined.[Method]Ten sweet corn inbred lines were tested on Pb and Cd accumulation.In a pot experiment,the heavy metals in roots,stems/leaves,and kernels of the plants grown on soil with spiked Pb and/or Cd were measured over time.Applicability of a molecular detection method on Pb and Cd at the InDel locus of ZmHMA2 in corn plant was scrutinized.[Result]The accumulation of Pb and Cd in organs of a corn plant was roots＞stems/leaves＞kernels.It did not differ significantly whether the metal elements were presented individually or simultaneously in the soil.Two inbred lines were identified to retain in the kernels less on Pb,3 on Cd,and only Mintian X901 on both Pb and Cd.The targeted molecular detection found InDel 2307 locus in 4 of the specimens,the average content of Cd which contained were 1.801 mg·kg-1 lower in the roots,0.64 mg·kg-1 lower in the stems/leaves,and 0.131 mg·kg-1 lower in the kernels compared to the average of the other varieties.[Conclusion]The InDel 2307 locus as a marker displayed a specificity in differentiating Cd content in corn plants grown on soils contaminated with different levels of the heavy metals.The molecular detection result and the Pb and Cd contents on the 10 sweet corn inbred lines indicated that Mintian X901 was the cultivar least prone to accumulate the pollutants.关键词
重金属/Pb污染/Cd污染/甜玉米/积累特征/分子检测Key words
Heavy metal/Pb pollution/Cd pollution/sweet corn/accumulation/marker detection分类
农业科技引用本文复制引用
李淑君,刘娅娟,张扬,蒲汝民,袁亮,廖长见..甜玉米自交系重金属Pb、Cd积累差异及分子检测[J].福建农业学报,2024,39(3):251-258,8.基金项目
重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0226) （cstc2021jcyj-msxmX0226）
福建省农业科学院自由探索项目(ZYTS2023004) （ZYTS2023004）
