福建农业学报2024，Vol.39Issue(3)：339-344,6.DOI:10.19303/j.issn.1008-0384.2024.03.011
柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用
A Multiplex RT-PCR Assay for Detecting Three Pathogens Infecting Citrus Plants
摘要
Abstract
[Objective]A multiplex RT-PCR assay to simultaneously detect citrus yellow vein clearing virus(CYVCV),citrus tristeza virus(CTV),and hop stunt viroid(HSVd)was developed.[Method]Multiplex RT-PCR primers were designed,and their specificity was analyzed.The optimal concentration ratio,annealing temperature and sensitivity of primers were determined.Moreover,citrus samples from Fujian region were detected by the established multiplex RT-PCR.[Result]The optimized concentration ratio of CYVCV-F/R,CTV-F/R,and HSVd-F/R primers was 1:1:2,and the annealing temperature at 52.9℃for the assay.The assay displayed a sensitivity at 10-2 dilution and the positive detections on CYVCV of 47.1%,on CTV of 56.7%,and on HSVd of 22.9%on 157 detected samples.[Conclusion]A rapid,accurate,applicable multiplex RT-PCR method for CYVCV,CTV,and HSVd detections was successfully established.关键词
柑橘黄化脉明病毒/柑橘衰退病毒/啤酒花矮化类病毒/多重RT-PCRKey words
Citrus yellow vein clearing virus/citrus tristeza virus/hop stunt viroid/multiplex RT-PCR分类
农业科技引用本文复制引用
袁琳凯,马崇欢,李丁山,陈志炜,江宵烽,丁新伦,张洁,吴祖建..柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用[J].福建农业学报,2024,39(3):339-344,6.基金项目
福建省三明市横向项目(KH210264A、KH210295A、KH220062A) （KH210264A、KH210295A、KH220062A）
