福建农业学报2024，Vol.39Issue(3)：362-368,7.DOI:10.19303/j.issn.1008-0384.2024.03.014
表面增强拉曼光谱法快速检测水产品中恩诺沙星和环丙沙星残留
SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood
摘要
Abstract
[Objective]A rapid method for detecting residues of enrofloxacin(ENR)and ciprofloxacin(CIP)in seafood by surface-enhanced Raman spectroscopy(SERS)was developed.[Method]On spiked seafood samples,(50±5)nm silver nanoparticles were used as the enhancement reagent and 200 g·L-1 NaCl solution as the extraction solution for the SERS assay.Detection results were read within 3 min and mathematical correlation between peak measurement and drug concentration examined.Based on the linearity and limit of detection,optimal assay conditions were determined.[Result]The assay showed several peaks on ENR and CIP standards.The C-N bending vibration caused the peak at 532 cm-1,the out-of-plane bending vibration at 552 cm-1,the C-N,C-C-F out-of-plane and C-C=O in-plane bending vibrations at 651 cm-1,the stretching vibration of C-H on the benzene ring,C-C=O out-of-plane bending vibration,C-C-N bending vibration and C-H,C-N stretching vibration at 737 cm-1,and the C-H,C-N out-of-plane bending vibration at 785 cm-1.By quantifying the characteristic peak at 737 cm-1,a linear relationship with an R2＞0.96 was found between the Raman intensity and drug concentration in the range of 10-200 ng·mL-1.The assay had a recovery rate of 78.4%～106.7%,RSD of 2.1%～6.7%,and a detection limit of 10μg·kg-1.[Conclusion]The newly developed rapid SERS assay for detecting ENR and CIP residues in seafood was deemed accurate,stable,and applicable for field screening and testing.关键词
表面增强拉曼光谱/恩诺沙星/环丙沙星/快速检测Key words
Surface-enhanced Raman spectroscopy/enrofloxacin/ciprofloxacin/rapid detection分类
轻工纺织引用本文复制引用
刘文静,潘葳,林惠真,陈宏炬,严绍德..表面增强拉曼光谱法快速检测水产品中恩诺沙星和环丙沙星残留[J].福建农业学报,2024,39(3):362-368,7.基金项目
福建省科技计划公益类专项(2021R1022008) （2021R1022008）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021020) （XTCXGC2021020）
福建省农业科学院创新团队建设项目(CXTD2021011-1) （CXTD2021011-1）
