分析测试学报2024，Vol.43Issue(6)：805-813,9.DOI:10.12452/j.fxcsxb.24030805
畜禽组织中甲氧苄啶和氟苯尼考的同时免疫层析检测方法研究
Simultaneous Detection of Trimethoprim and Florfenicol in Livestock Tissues Using Immunochromatography
摘要
Abstract
To address the problem of residues of trimethoprim(TMP)and florfenicol(FF)used in combination with food of animal origin,a latex microsphere lateral flow immunochromatographic as-say(LMs-LFIA)for the simultaneous detection of trimethoprim and florfenicol in chicken and pork samples using latex microspheres as tracer signal probes was established.Based on the specific mono-clonal antibodies 7E6 and SF15,a stepwise optimization strategy investigated the effects of probe preparation conditions,test strip working buffer,and sample pad treatment system on the perfor-mance of LMs-LFIA.The results showed that under the optimal conditions,the established LMs-LFIA visualized the detection limits of trimethoprim and florfenicol in chicken and pork samples at 8 ng/g and 12 ng/g,respectively,with a detection time of 8 min.There was no obvious crossover be-tween trimethoprim and florfenicol's structural and functional analogs,indicating good specificity.The established method was used for blind detection of chicken and pork samples,and the results were in agreement with those obtained by the UPLC-MS/MS instrumental confirmatory method.Therefore,the method is suitable for the simultaneous detection of trimethoprim and florfenicol resi-dues in livestock and poultry tissue samples.关键词
甲氧苄啶/氟苯尼考/单克隆抗体/乳胶微球侧流免疫层析/快速检测Key words
trimethoprim/florfenicol/monoclonal antibodies/latex microspheres lateral flow im-munochromatographic assay(LMs-LFIA)/rapid detection分类
化学化工引用本文复制引用
高亚晖,张咏仪,凌志洲,谢美婵,杨金易,徐振林,王弘,沈玉栋..畜禽组织中甲氧苄啶和氟苯尼考的同时免疫层析检测方法研究[J].分析测试学报,2024,43(6):805-813,9.基金项目
国家自然科学基金项目(32272400) （32272400）
广州市科技计划项目(202206010146) （202206010146）
