分析测试学报2024，Vol.43Issue(6)：826-836,11.DOI:10.12452/j.fxcsxb.24011501
佩兰的HPLC特征图谱建立及化学成分鉴定
HPLC Characteristic Chromatogram Establishment and Chemical Components ldentification of Eupatorium Fortunei
摘要
Abstract
A high performance liquid chromatography(HPLC)characteristic chromatogram analysis method of Eupatorium fortunei(E.fortunei)was established to differentiate it from its adulterants.Ul-trahigh performance liquid chromatography-Q-exactive orbitrap mass spectrometry(UHPLC-Q-Exac-tive Orbitrap MS)was operated under positive and negative ion mode to collect the data of total ions current(TIC).The ion source was a heated electrospray ionization(HESI)source.According to the accurate mass,fragment ions was combined with literature information,compound database and the reference substances were identified to conduct a preliminary identification of the chemical compo-nents in E.fortunei.The chromatographic separation was carried out on a Waters HSS T3(250 mm×4.6 mm,5.0 μm)column,using acetonitrile-0.1%phosphoric acid as mobile phase by gradient elution.The collected HPLC data analyzed by a similar evaluation system for chromatographic finger-print of traditional Chinese medicine(version 2012,National Committee of Pharmacopoeia,China).HPLC charastersitic chromatograms of 23 batches of E.fortunei were established,facilitating a com-parative assessment between the characteristic chromatograms of genuine samples and its respective adulterants,and hierarchical cluster analysis(HCA)was used to verify the common peaks area as the variable.Results showed that a total of 34 chemical components were successfully identified within E.fortunei,including 11 phenylpropanoids,6 pyrrolizidine alkaloids,3 nucleosides,3 amino ac-ids,2 flavonoids,9 other glycoside,acid,alkali,ketone and aldehyde compounds,and the au-thenticity of 8 components were confirmed through comparison with the reference substance.There are 16 common peaks across the charastersitic chromatograms of 23 batches of E.fortunei and the similarities are greater than 0.85.The similarities between the chromatograms of the adulterants and the reference chromatogram of E.fortunei was 0.452-0.746.According to the area of common peaks,E.fortunei and its adulterants were clearly classified through hierarchical cluster analysis.The established HPLC characteristic chromatograms method of E.fortunei had good specificity,pre-cision,repeatability,stability,and components identification results not only serve as a valuable reference for quality control of E.fortunei but also prove highly effective in the reliable differentiation of E.fortunei and its adulterants,ensuring the quality and efficacy of E.fortunei is of significant im-portance.At the same time,this research also lays the foundation for further in-depth studies on the chemical composition and pharmacological effects of E.fortunei.关键词
佩兰/特征图谱/真伪鉴别/成分分析/UHPLC-Q-Exactive Orbitrap MSKey words
Eupatorium fortunei/characteristic chromatograms/authenticity identification/com-ponent analysis/UHPLC-Q-Exactive Orbitrap MS分类
化学化工引用本文复制引用
谢翡翡,卢晓莹,叶聪,吴润松,罗睿,何广铭,李振雨,罗文汇..佩兰的HPLC特征图谱建立及化学成分鉴定[J].分析测试学报,2024,43(6):826-836,11.基金项目
国家工信部2022年产业技术基础公共服务平台项目-中药全产业链质量技术服务平台(2022-230-221) （2022-230-221）
2022年佛山市南海区重点领域科技攻关专项(南科[2023]20号-18) （南科[2023]20号-18）
