分析测试学报2024，Vol.43Issue(6)：837-847,11.DOI:10.12452/j.fxcsxb.24022103
渗透汽化结合GC-MS分析豫烟浸膏的风味组分
Analysis of Flavor Components in Yuyan Extract Based on Pervaporation and GC-MS
摘要
Abstract
In order to investigate the effect of pervaporation on the extraction of volatile components from Yuyan extract,solid-phase supported liquid-liquid extraction/gas chromatography‒mass spec-trometry(SLE/GC‒MS)and sensory characteristics quantitative description method were used to ana-lyze the separated sample solution for non-targeted analysis and aroma characteristics evaluation.And stoichiometry was used to explore the difference of sample components,screen the key difference components and analyze their correlation with sensory aroma characteristics.The results showed that:(1)Compared with the original solution,the sample solution obtained by pervaporation separation under the optimized working conditions was significantly improved in the style of fruit aroma,fra-grance and sweet aroma,and the paste aroma was weakened,in which the aroma attribute score of the intercepted solution was improved as a whole,and the permeate and cleaning solution were slight-ly weakened;(2)A total of 101 compounds were detected in different sample solutions,including 13 kinds of substances,such as alcohols,esters,aldehydes,ketones,acids,phenols,hydrocar-bons and others mainly composed of alkaloids.Hydrocarbons,ketones and others accounted for a large proportion of volatile components.The membrane components had greater selectivity for hydro-carbons,alcohols and other components,and different solvent systems had greater influence on the concentration of alcohols,ketones,esters and phenols;(3)Principal component analysis showed that the components of sample stock solution and retention solution were relatively consistent,and they were effectively distinguished from permeating solution and cleaning solution.Orthogonal partial least squares discriminant analysis screened 28 different compounds(VIP>1,P<0.05).Partial least squares regression analysis showed that the characteristic volatile components were positively correlat-ed with the aroma indexes of flue-cured tobacco,such as tobacco aroma,wood aroma,fragrance,sweet aroma and burnt aroma.The results provide guidance for the extraction of Yuyan extract by per-vaporation,and then provide technical support for the fine separation and processing of natural plant aroma raw materials.关键词
渗透汽化/豫烟浸膏/气相色谱‒质谱联用/风味组分/感官特征/化学计量学Key words
pervaporation/Yuyan extract/gas chromatography-mass spectrometry/flavor compo-nents/sensory characteristics/chemometrics分类
化学化工引用本文复制引用
窦思颖,张华,沈海峰,朱怀远,姚忠,廖惠云..渗透汽化结合GC-MS分析豫烟浸膏的风味组分[J].分析测试学报,2024,43(6):837-847,11.基金项目
江苏中烟工业有限责任公司对外合作科技创新项目(H202111) （H202111）
中国烟草总公司科技项目(110202202024) （110202202024）
