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首页|期刊导航|分析测试学报|基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对天然牛黄和体外培育牛黄胆汁酸类成分的差异性分析

基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对天然牛黄和体外培育牛黄胆汁酸类成分的差异性分析OA北大核心CSTPCD

Differential Analysis of Bile Acid Components in Bovis Calculus and Bovis Calculus Sativus Based on UHPLC-QE-Orbitrap MS

中文摘要英文摘要

采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-QE-Orbitrap MS)对天然牛黄和体外培育牛黄共18批样品进行分析,使用Compound Discoverer软件对胆汁酸类成分进行鉴定,结合化学统计学分析筛选不同类型牛黄的差异性成分.根据一级质谱精确质荷比和二级化合物碎片信息,鉴定了30个共有的胆汁酸类成分.聚类分析结果表明体外培育牛黄与产地呈现一致趋势,而天然牛黄的聚类趋势与产地无关.主成分分析载荷图显示胆酸、去氧胆酸等成分对主成分1的贡献较大,而甘氨鹅去氧胆酸、甘氨去氧胆酸、牛磺去氧胆酸等成分对主成分2的贡献较大.通过建立正交偏最小二乘法判别(OPLS-DA)模型,共筛选出11个胆汁酸类差异成分(VIP>1,P<0.05),其中胆酸在两组之间的含量差异最大.除了去氢胆酸和甘氨去氢胆酸,其余9个差异成分在体外培育牛黄中的相对含量均较高.研究结果可为天然牛黄和体外培育牛黄的物质基础、质量控制和资源开发应用提供理论参考.

An ultra-high performance liquid chromatography-Q-Exactive-orbitrap mass spectromet-ric(UHPLC-QE-Orbitrap MS)method was used to analyse 18 batches of Bovis Calculus(BC)and Bovis Calculus Sativus(BCS)samples.Compound Discoverer software was used to identify the bile acid components,and the chemical statistical analysis was used to screen the differential components from different types of BC samples.The results showed that 30 bile acid components were identified based on the accurate m/z ratio data and the fragment informations from mass spectrometry.Cluster-ing analysis results indicate that the trend of BCS is consistent with its place of production,while the trend of BC is independent of its source.The load plot of principal component analysis shows that cho-lic acid and deoxycholic acid contribute significantly to the principal component 1,while glycocheno-deoxycholic acid,glycodeoxycholic acid,and taurodeoxycholic acid contribute significantly to the principal component 2.By establishing an OPLS-DA model,11 differential bile acid components were selected(VIP>1,P<0.05),and the difference of cholic acid content is the greatest between two groups.Except dehydrocholic acid and glycodehydrocholic acid,the relative content of the re-maining 9 differential components in BCS is higher.This research provide a reference for the material basis,quality control,resource development and application of BC and BCS.

李沁;陈三理;王倩;陈海滨;金鸣;陈在敏;吴丽璇

福建省食品药品质量检验研究院,福建 福州 350012漳州片仔癀药业股份有限公司,福建 漳州 363099

化学

UHPLC-QE-Orbitrap MS天然牛黄体外培育牛黄胆汁酸差异性分析

UHPLC-QE-orbitrap MSBovis CalculusBovis Calculus Sativusbile aciddiffer-ential analysis

《分析测试学报》 2024 (006)

891-897 / 7

10.12452/j.fxcsxb.24020401

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