分析测试学报2024，Vol.43Issue(6)：891-897,7.DOI:10.12452/j.fxcsxb.24020401
基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对天然牛黄和体外培育牛黄胆汁酸类成分的差异性分析
Differential Analysis of Bile Acid Components in Bovis Calculus and Bovis Calculus Sativus Based on UHPLC-QE-Orbitrap MS
李沁 1陈三理 1王倩 2陈海滨 1金鸣 1陈在敏 1吴丽璇2
作者信息
- 1. 福建省食品药品质量检验研究院,福建 福州 350012
- 2. 漳州片仔癀药业股份有限公司,福建 漳州 363099
- 折叠
摘要
Abstract
An ultra-high performance liquid chromatography-Q-Exactive-orbitrap mass spectromet-ric(UHPLC-QE-Orbitrap MS)method was used to analyse 18 batches of Bovis Calculus(BC)and Bovis Calculus Sativus(BCS)samples.Compound Discoverer software was used to identify the bile acid components,and the chemical statistical analysis was used to screen the differential components from different types of BC samples.The results showed that 30 bile acid components were identified based on the accurate m/z ratio data and the fragment informations from mass spectrometry.Cluster-ing analysis results indicate that the trend of BCS is consistent with its place of production,while the trend of BC is independent of its source.The load plot of principal component analysis shows that cho-lic acid and deoxycholic acid contribute significantly to the principal component 1,while glycocheno-deoxycholic acid,glycodeoxycholic acid,and taurodeoxycholic acid contribute significantly to the principal component 2.By establishing an OPLS-DA model,11 differential bile acid components were selected(VIP>1,P<0.05),and the difference of cholic acid content is the greatest between two groups.Except dehydrocholic acid and glycodehydrocholic acid,the relative content of the re-maining 9 differential components in BCS is higher.This research provide a reference for the material basis,quality control,resource development and application of BC and BCS.关键词
UHPLC-QE-Orbitrap MS/天然牛黄/体外培育牛黄/胆汁酸/差异性分析Key words
UHPLC-QE-orbitrap MS/Bovis Calculus/Bovis Calculus Sativus/bile acid/differ-ential analysis分类
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李沁,陈三理,王倩,陈海滨,金鸣,陈在敏,吴丽璇..基于超高效液相色谱-四极杆-静电场轨道阱高分辨质谱对天然牛黄和体外培育牛黄胆汁酸类成分的差异性分析[J].分析测试学报,2024,43(6):891-897,7.
